Analysis of four achaete-scute homologs in Bombyx mori reveals new viewpoints of the evolution and functions of this gene family

Qingxiang Zhou; Tianyi Zhang; Weihua Xu; Linlin Yu; Yongzhu Yi; Zhifang Zhang
January 2008
BMC Genetics;2008, Vol. 9, Special section p1
Academic Journal
Background: achaete-scute complexe (AS-C) has been widely studied at genetic, developmental and evolutional levels. Genes of this family encode proteins containing a highly conserved bHLH domain, which take part in the regulation of the development of central nervous system and peripheral nervous system. Many AS-C homologs have been isolated from various vertebrates and invertebrates. Also, AS-C genes are duplicated during the evolution of Diptera. Functions besides neural development controlling have also been found in Drosophila AS-C genes. Results: We cloned four achaete-scute homologs (ASH) from the lepidopteran model organism Bombyx mori, including three proneural genes and one neural precursor gene. Proteins encoded by them contained the characteristic bHLH domain and the three proneural ones were also found to have the C-terminal conserved motif. These genes regulated promoter activity through the Class A E-boxes in vitro. Though both Bm-ASH and Drosophila AS-C have four members, they are not in one by one corresponding relationships. Results of RT-PCR and real-time PCR showed that Bm-ASH genes were expressed in different larval tissues, and had well-regulated expressional profiles during the development of embryo and wing/wing disc. Conclusion: There are four achaete-scute homologs in Bombyx mori, the second insect having four AS-C genes so far, and these genes have multiple functions in silkworm life cycle. AS-C gene duplication in insects occurs after or parallel to, but not before the taxonomic order formation during evolution.


Related Articles

  • Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Tatematsu, Ken-ichiro; Kobayashi, Isao; Uchino, Keiro; Sezutsu, Hideki; Iizuka, Tetsuya; Yonemura, Naoyuki; Tamura, Toshiki // Transgenic Research;Jun2010, Vol. 19 Issue 3, p473 

    To construct an efficient system for the production of recombinant proteins in silkworm ( Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes ( Ser1, Ser2, and Ser3) using a GAL4/ UAS binary gene expression system in transgenic silkworm. The promoter...

  • An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Wang, Feng; Xu, Hanfu; Yuan, Lin; Ma, Sanyuan; Wang, Yuancheng; Duan, Xiaoli; Duan, Jianping; Xiang, Zhonghuai; Xia, Qingyou // Transgenic Research;Oct2013, Vol. 22 Issue 5, p925 

    The middle silk gland (MSG) of silkworm is thought to be a potential host for mass-producing valuable recombinant proteins. Transgenic MSG expression systems based on the usage of promoter of sericin1 gene (sericin-1 expression system) have been established to produce various recombinant...

  • The right size for a mammal. Purvis, Andy; Harvey, Paul H. // Nature;3/27/1997, Vol. 386 Issue 6623, p332 

    Discusses a model of life-history evolution for animals that stop growing when they reach reproductive maturity proposed by Kozlowski and Weiner. The relationships between life-history attributes and body size among species; The paper in a 1997 issue of `American Naturalist.'

  • Phylogenetic analyses with proximal promoter from skeletal and cardiac calsequestrins. BAKIU, RIGERS; VALLE, GIORGIA; NORI, ALESSANDRA // Albanian Journal of Agricultural Sciences;2013, Vol. 12 Issue 4, p575 

    Calsequestrin (CASQ) is the main calcium binding protein of the sarcoplasmic reticulum. In mammalian muscles, it exists as a skeletal isoform (CASQ1) found in fast- and slow-twitch skeletal muscles and a cardiac isoform (CASQ2) expressed in the heart and slow-twitch muscles. Evolutionary biology...

  • EVOLUTION: Same rates for neighbours. Casci, Tanita // Nature Reviews Genetics;Dec2000, Vol. 1 Issue 3, p163 

    Discusses a study on the rates of evolution to proteins encoded by neighboring genes. Data from linked mouse-rat orthologous genes; Correlation between the evolution rate of linked proteins and the evolution rate of their encoding genes; Reasons for the co-evolution of proteins encoded by...

  • An efficient microinjection method for unfertilized eggs of Asian amphioxus Branchiostoma belcheri. Liu, Xin; Li, Guang; Feng, Jun; Yang, Xi; Wang, Yi-Quan // Development Genes & Evolution;Jul2013, Vol. 223 Issue 4, p269 

    Amphioxus is a promising model animal for evolutionary and developmental studies. However, as an emerging model organism, amphioxus lacks most molecular techniques applied in other well-developed model animals. Microinjection is a powerful technique for gene manipulation, and thus it undoubtedly...

  • Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3. Kimoto, Mai; Kitagawa, Tsuyuki; Kobayashi, Isao; Nakata, Tomohiro; Kuroiwa, Asato; Takiya, Shigeharu // Development Genes & Evolution;Nov2012, Vol. 222 Issue 6, p351 

    The sericin-1 gene encoding a glue protein is expressed in the middle silk gland (MSG) of the silkworm, Bombyx mori. A member of the class III POU domain transcription factors, POU-M1, was cloned as the factor bound to the SC site of the sericin-1 promoter and has been proposed to be a positive...

  • Continuous evolution: Protein evolution at warp speed. Lehman, Niles // Nature Chemical Biology;May2011, Vol. 7 Issue 5, p252 

    The article focuses on phage-assisted continuous evolution (PACE) experiments produced unforeseen and improved RNAs and DNAs. The application of PACE is used to create enzyme functions in three situations such as the adjustment of T7 RNA polymerase to use T3 promoter sequence and the...

  • Subfamily Limoniinae Speiser, 1909 (Diptera, Limoniidae) from Baltic Amber (Eocene): The Genus Elephantomyia Osten Sacken, 1860. Kania, Iwona // PLoS ONE;Feb2015, Vol. 10 Issue 2, p1 

    A revision of the genus Elephantomyia Osten Sacken (Diptera: Limoniidae) from Baltic amber (Eocene) is presented. Four species—E. baltica Alexander, E. brevipalpa Loew, E. longirostris Loew, and E. pulchella Loew—are redescribed and documented with photographs and drawings. In...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics