Botulinum toxin detection using AlGaN/GaN high electron mobility transistors

Wang, Yu-Lin; Chu, B. H.; Chen, K. H.; Chang, C. Y.; Lele, T. P.; Tseng, Y.; Pearton, S. J.; Ramage, J.; Hooten, D.; Dabiran, A.; Chow, P. P.; Ren, F.
December 2008
Applied Physics Letters;12/29/2008, Vol. 93 Issue 26, p262101
Academic Journal
Antibody-functionalized, Au-gated AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect botulinum toxin. The antibody was anchored to the gate area through immobilized thioglycolic acid. The AlGaN/GaN HEMT drain-source current showed a rapid response of less than 5 s when the target toxin in a buffer was added to the antibody-immobilized surface. We could detect a range of concentrations from 1 to 10 ng/ml. These results clearly demonstrate the promise of field-deployable electronic biological sensors based on AlGaN/GaN HEMTs for botulinum toxin detection.


Related Articles

  • Llamas: Soldiers in the War on Terror.  // Airport Security Report;2/10/2010, Vol. 18 Issue 3, p9 

    The article reports on the detection of botulinum neurotoxins (BoNTs) through the BoNT-detecting substances called antibodies-proteins which are found in llamas. It is noted that BoNTs are about 100 billion times more toxic that cyanide and are the potential bioterror threats. It further states...

  • Detection of Botulinum Neurotoxin by Tapered Fiber Optic Biosensor. Zhijing (Jennifer) Guo; Boyter, Chris; Cohoon, Greg; Salik, Ertan; Wei-Jen Lin // GSTF International Journal on Bioinformatics & Biotechnology;Dec2011, Vol. 1 Issue 1, p39 

    Botulinum neurotoxin (BoNT) is the most poisonous protein known so far, with a minimal lethal dose of 1 ng/kg for humans. Current in vitro detection methods for BoNTs are time consuming and lack sensitivity. Our goal is to develop a highly sensitive, label-free sensor for BoNT detection using...

  • [Non)inferiority complex. Nataloni, Rochelle // Cosmetic Surgery Times;Aug2012, Vol. 15 Issue 7, p10 

    The article discusses the botulinum toxin which has both cosmetic and therapeutic applications. It informs that xeomin is used for temporary improvement in the appearance of moderate-to-severe glabellar lines. It has been found that incobotulinumtoxin A is less immunogenic and sometimes results...

  • Managing Adverse Events Associated with Botulinum Toxin Type A: A Focus on Cosmetic Procedures. Wollina, Uwe; Konrad, Helga // American Journal of Clinical Dermatology;2005, Vol. 6 Issue 3, p141 

    Botulinum toxin A (BTXA) has become a widely used drug in cosmetic dermatology, not only to treat focal hyperhidrosis but also hyperkinetic facial lines, platysma bands, décolleté bands, and other skin features. The spectrum of possible adverse effects of BTXA is broad but fortunately...

  • Inhibition of Botulinum Neurotoxin A Toxic Action In Vivo by Synthetic Synaptosome- and Blocking Antibody-Binding Regions. Atassi, M. Zouhair; Dolimbek, Behzod Z.; Steward, Lance E.; Aoki, K. Roger // Protein Journal;Jul2010, Vol. 29 Issue 5, p320 

    In previous studies, we showed that certain peptides of the HN and HC domains of the H-chain of BoNT/A bind to mouse brain synaptosomes (snps). There was either complete correspondence or overlap between peptides that bind snps and those that bind human or mouse blocking antibodies (Abs). An...

  • Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating. Lou, J.; Geren, I.; Garcia-Rodriguez, C.; Forsyth, C. M.; Wen, W.; Knopp, K.; Brown, J.; Smith, T.; Smith, L. A.; Marks, J. D. // PEDS: Protein Engineering, Design & Selection;Apr2010, Vol. 23 Issue 4, p311 

    Botulism is caused by the botulinum neurotoxins (BoNTs), the most poisonous substance known. Because of the high potency of BoNT, development of diagnostic and therapeutic antibodies for botulism requires antibodies of very high affinity. Here we report the use of yeast mating to affinity mature...

  • Llama-derived single-domain antibodies for the detection of botulinum A neurotoxin. Swain, Marla D.; Anderson, George P.; Zabetakis, Dan; Bernstein, Rachael D.; Liu, Jinny L.; Sherwood, Laura J.; Hayhurst, Andrew; Goldman, Ellen R. // Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 1, p339 

    Single-domain antibodies (sdAb) specific for botulinum neurotoxin serotype A (BoNT A) were selected from an immune llama phage display library derived from a llama that was immunized with BoNT A toxoid. The constructed phage library was panned using two methods: panning on plates coated with...

  • Development of Human-Like scFv-Fc Neutralizing Botulinum Neurotoxin E. Miethe, Sebastian; Rasetti-Escargueil, Christine; Avril, Arnaud; Liu, Yvonne; Chahboun, Siham; Korkeala, Hannu; Mazuet, Christelle; Popoff, Michel-Robert; Pelat, Thibaut; Thullier, Philippe; Sesardic, Dorothea; Hust, Michael // PLoS ONE;10/6/2015, Vol. 10 Issue 10, p1 

    Background: Botulinum neurotoxins (BoNTs) are considered to be the most toxic substances known on earth and are responsible for human botulism, a life-threatening disease characterized by flaccid muscle paralysis that occurs naturally by food-poisoning or colonization of the gastrointestinal...

  • Aptamer-based electrochemical biosensor for Botulinum neurotoxin. Fang Wei; Chih-Ming Ho // Analytical & Bioanalytical Chemistry;Apr2009, Vol. 393 Issue 8, p1943 

    We have developed an aptamer-based electrochemical sensor for detection of Botulinum neurotoxin, where steric hindrance is applied to achieve specific signal amplification via conformational change of the aptamer. The incubation time and potassium concentration of the reaction buffer were found...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics