TITLE

Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression

AUTHOR(S)
Carvalho, B.; Postma, C.; Mongera, S.; Hopmans, E.; Diskin, S.; van de Wiel, M. A.; van Criekinge, W.; Thas, O.; Matthäi, A.; Cuesta, M. A.; sive Droste, J. S. Terhaar; Craanen, M.; Schröck, E.; Ylstra, B.; Meijer, G. A.
PUB. DATE
January 2009
SOURCE
Gut;Jan2009, Vol. 58 Issue 1, p79
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: This study aimed to identify the oncogenes at 20q involved in colorectal adenoma to carcinoma progression by measuring the effect of 20q gain on mRNA expression of genes in this amplicon. Methods: Segmentation of DNA copy number changes on 20q was performed by array CGH (comparative genomic hybridisation) in 34 non-progressed colorectal adenomas, 41 progressed adenomas (ie, adenomas that present a focus of cancer) and 33 adenocarcinomas. Moreover, a robust analysis of altered expression of genes in these segments was performed by microarray analysis in 37 adenomas and 31 adenocarcinomas. Protein expression was evaluated by immunohistochemistry on tissue microarrays. Results: The genes C20or124, AURKA, RNPCI, TH1L, AURM1, C20orf20 and TCFL5, mapping at 20q, were significantly overexpressed in carcinomas compared with adenomas as a consequence of copy number gain of 20q. Conclusion: This approach revealed C20orf24, AURKA, RNPCI, TH1L, ADRM1, C20orf20 and TCFL5 genes to be important in chromosomal instability-related adenoma to carcinoma progression. These genes therefore may serve as highly specific biomarkers for colorectal cancer with potential clinical applications.
ACCESSION #
35965712

 

Related Articles

  • Targeting the translation machinery in cancer. Bhat, Mamatha; Robichaud, Nathaniel; Hulea, Laura; Sonenberg, Nahum; Pelletier, Jerry; Topisirovic, Ivan // Nature Reviews Drug Discovery;Apr2015, Vol. 14 Issue 4, p261 

    Dysregulation of mRNA translation is a frequent feature of neoplasia. Many oncogenes and tumour suppressors affect the translation machinery, making aberrant translation a widespread characteristic of tumour cells, independent of the genetic make-up of the cancer. Therefore, therapeutic agents...

  • Silencing Human Cancer: Identification and Uses of MicroRNAs. Nicolas, Francisco E.; Lopez-Gomollon, Sara; Lopez-Martinez, Alfonso F.; Dalmay, Tamas // Recent Patents on Anti-Cancer Drug Discovery;Jan2011, Vol. 6 Issue 1, p94 

    MicroRNAs (miRNAs) are a new class of negative regulators that repress gene expression by pairing with their target messenger RNAs (mRNAs). There are hundreds of miRNAs coded in the human genome and thousands of target mRNAs participating in a wide variety of physiological processes such as...

  • Nm23-H1 expression and loss of heterozygosity in colon adenocarcinoma. Kapitanovic, S.; Cacev, T.; Berkovic, M.; Popovic-Had�ija, M.; Rado�Evc, S.; Seiwerth, S.; Spaventi, �.; Povelic, K.; Spaventi, R. // Journal of Clinical Pathology;Nov2004, Vol. 57 Issue 11, p1312 

    Background: The discovery that genetic alterations in oncogenes and tumour suppressor genes accompany tumour formation in many human tumours has encouraged the search for genes that promote or suppress tumour spread and metastasis; nm23 is a promising candidate for a metastasis suppressing gene....

  • c-erbB2 and c-myb Induce Mouse Oocyte Maturation Involving Activation of Maturation Promoting Factor. Zheng, Li-Ping; Huang, Jian; Zhang, Da-Lei; Xu, Liang-Quang; Li, Fang; Wu, Lei; Liu, Zheng-Yu; Zheng, Yue-Hui // DNA & Cell Biology;Feb2012, Vol. 31 Issue 2, p164 

    Proto-oncogenes are involved in cell growth, proliferation, and differentiation. In the present study, we investigated the roles and mediating pathways of proto-oncogenes c-erbB2 and c-myb in mouse oocyte maturation by RT-PCR, real-time quantitative PCR, western blot, and recombinant...

  • Polyadenylation: alternative lifestyles of the A-rich (and famous?). Dickson, Alexa M; Wilusz, Jeffrey // EMBO Journal;5/5/2010, Vol. 29 Issue 9, p1473 

    The article discusses various topics published within the issue including one about the A-rich sequence element, one about the development of oncogene activation, and another one regarding the end processing of mRNA.

  • JournalScan.  // Gut;Jun2005, Vol. 54 Issue 6, p885 

    The article presents abstracts for various papers related to gastroenterology and hepatology. It informs about a study in which the author first studied 684 patients undergoing total colonoscopy twice within 30 days to calculate odds ratio of missing neoplastic polyps and the relative risk for...

  • MicroRNAs in Human Embryonic and Cancer Stem Cells. Navarro, Alfons; Monzó, Mariano // Yonsei Medical Journal;9/1/2010, Vol. 51 Issue 5, p622 

    MicroRNAs (miRNAs) are small non-coding RNAs that regulate messenger RNAs at the post-transcriptional level. They play an important role in the control of cell physiological functions, and their alterations have been related to cancer, where they can function as oncogenes or tumor suppressor...

  • miR-21 and 221 upregulation and miR-181b downregulation in human grade II—IV astrocytic tumors. Alfredo Conti; M’Hammed Aguennouz; Domenico La Torre; Chiara Tomasello; Salvatore Cardali; Filippo Angileri; Francesca Maio; Annamaria Cama; Antonino Germanò; Giuseppe Vita; Francesco Tomasello // Journal of Neuro-Oncology;Jul2009, Vol. 93 Issue 3, p325 

    Abstract  MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. Recent evidence indicates that miRNAs can function both as tumor suppressors and as oncogenes. It has been demonstrated that...

  • Detection of novel mRNA splice variants of human ING4 tumor suppressor gene. Raho, G.; Miranda, C.; Tamborini, E.; Pierotti, M. A.; Greco, A. // Oncogene;8/9/2007, Vol. 26 Issue 36, p5247 

    Inhibitor of growth (ING)4, member of a gene family encoding potential tumor suppressors, is implicated as a repressor of angiogenesis and tumor growth and suppresses loss of contact inhibition in vitro. Here, we report that ING4 undergoes alternative splicing. Expression analysis identified...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics