TITLE

VIRAL DELIVERY OF SMALL-HAIRPIN RNAS FOR REDUCING GENE EXPRESSION IN THE RODENT BRAIN

AUTHOR(S)
Lasek, Amy W.; Heberlein, Ulrike
PUB. DATE
September 2008
SOURCE
Alcohol Research & Health;2008, Vol. 31 Issue 3, p259
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A descibed in the preceding article by Adams and Zawada (pp. 256-258), short-interferring RNA (siRNA) molecules and other related RNA molecules cab be used successfully to disrupt the normal expression of specific genes in the mammalian brain—a process known as RNA interference (RNAi). However, although the strategy described by those authors has been shown to be effective, its usefuilness is limited by the fact that the observed effects are only transient. For some research questions, however, it is vital that expression of a specific gene is reduced (i.e., knocked down) for a longer period of time. This article reviews a strategy to ensure long-term expression of another type of interfering RNA molecule—that is, small-hairpin RNAs (shRNAs)— through the use of viral delivery systems (i.e., vectors). Expression of these shRNAs leads to the destruction of the intermediary molecules (i.e., messenger RNA [mRNA] molecules) generated during the expression of the target gene.
ACCESSION #
35876022

 

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