TITLE

Non-contact protein microarray fabrication using a procedure based on liquid bridge formation

AUTHOR(S)
Hartmann, Michael; Sjödahl, Johan; Stjernström, Mårten; Redeby, Johan; Joos, Thomas; Roeraade, Johan
PUB. DATE
January 2009
SOURCE
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p591
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Contemporary microarrayers of contact or non-contact format used in protein microarray fabrication still suffer from a number of problems, e.g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL–1 human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-μm polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.
ACCESSION #
35774258

 

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