Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Posthuma-Trumpie, Geertruida A.; Korf, Jakob; van Amerongen, Aart
January 2009
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p569
Academic Journal
Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for “strengths, weaknesses, opportunities, threats”). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included “immunochromatography”, “sol particle immunoassay”, “lateral flow immunoassay” and “dipstick assay”.


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