Speciation of arsenic trioxide metabolites in blood cells and plasma of a patient with acute promyelocytic leukemia

Yoshino, Yuta; Yuan, Bo; Miyashita, Shin-ich; Iriyama, Noriyoshi; Horikoshi, Akira; Shikino, Osamu; Toyoda, Hiroo; Kaise, Toshikazu
January 2009
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p689
Academic Journal
Arsenic trioxide (As2O3) has been widely accepted as the second-best choice for the treatment of relapsed and refractory acute promyelocytic leukemia (APL) patients. However, a few studies have been conducted on a detailed speciation of As2O3 metabolites in blood samples of patients. To clarify the speciation of arsenic, the blood samples were collected at various time points from a patient with APL after remission induction therapy and during consolidation therapy. The total amounts of arsenic in blood cells and plasma, and the plasma concentrations of inorganic arsenic and methylated metabolites were determined by inductively coupled plasma mass spectrometry (ICP-MS) and high-performance liquid chromatography/ICP-MS, respectively. The total amounts of arsenic in the blood cells were 4–10 times higher than those in plasma. Among all arsenic metabolites, the pentavalent arsenate (AsV) in plasma was more readily eliminated. During the drug-withdrawal period, the initial plasma concentrations of trivalent arsenic (AsIII) declined more rapidly than those of methylarsonic acid and dimethlyarsinic acid, which are known as the major methylated metabolites of AsIII. On the other hand, during the consecutive administration in the consolidation therapy period, the plasma concentrations of total arsenic and arsenic metabolites increased with time. In conclusion, these results may support the idea that methylated metabolites of As2O3 contribute to the efficacy of arsenic in APL patients. These results also suggest that detailed studies on the pharmacokinetics as well as the pharmacodynamics of As2O3 in the blood cells from APL patients should be carried out to provide an effective treatment protocol.


Related Articles

  • Speciated urinary arsenic as a biomarker of dietary exposure to inorganic arsenic in residents living in high-arsenic areas in Latium, Italy. Cubadda, Francesco; Aureli, Federica; D'Amato, Marilena; Raggi, Andrea; Turco, Anna Chiara; Mantovani, Alberto // Pure & Applied Chemistry;Feb2012, Vol. 84 Issue 2, p203 

    Current knowledge indicates that total urinary arsenic is not a suitable biomarker of exposure to toxic, i.e., inorganic, arsenic (iAs), whereas measurement of iAs and its methylated metabolites in urine using speciation analysis provides much more reliable estimates of exposure. The relative...

  • Mercury speciation analysis in human hair by species-specific isotope-dilution using GC-ICP-MS. Laffont, Laure; Maurice, Laurence; Amouroux, David; Navarro, Patricia; Monperrus, Mathilde; Sonke, Jeroen; Behra, Philippe // Analytical & Bioanalytical Chemistry;Mar2013, Vol. 405 Issue 9, p3001 

    We optimized a mercury (Hg) speciation extraction method for human hair in combination with species-specific isotope-dilution analysis by gas chromatography-inductively coupled plasma-mass spectrometry (GC-ICP-MS). The method was validated on human hair reference material RM (IAEA-086), which is...

  • Biological and chemical investigation of Allium cepa L. response to selenium inorganic compounds. Michalska-Kacymirow, M.; Kurek, E.; Smolis, A.; Wierzbicka, M.; Bulska, E. // Analytical & Bioanalytical Chemistry;Jun2014, Vol. 406 Issue 15, p3717 

    The aim of this study was to evaluate the biological and chemical response of Allium cepa L. exposed to inorganic selenium compounds. Besides the investigation of the total content of selenium as well as its chemical speciation, the Allium test was used to evaluate the growth of onion roots and...

  • Interface Development for Coupling Capillary Electrophoresis (CE) and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Application to Plutonium Speciation. Baglan, N.; Delorme, A.; Aupiais, J. // AIP Conference Proceedings;2003, Vol. 673 Issue 1, p393 

    Over the past few years, speciation studies have gained more and more popularity because its knowledge is necessary for understanding the transfer mechanism observed for various elements. Among them, radionuclide or chemical pollutants become a subject of interest, especially for predicting...

  • Epigenetics: an important challenge for ICP-MS in metallomics studies. Wrobel, Katarzyna; Wrobel, Kazimierz; Caruso, Joseph A. // Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p481 

    Trace metal analysis has been long regarded as one of the principle tasks in areas of chemical analysis. At the early stage of instrumental development, total concentration was assessed in a variety of samples, yielding results, among others, for environmental, biological, and clinical samples....

  • Some pitfalls in PAGE-LA-ICP-MS for quantitative elemental speciation of dissolved organic matter and metalomics. Jiménez, Maria S.; Gomez, Maria T.; Rodriguez, L.; Martinez, L.; Castillo, Juan R. // Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p699 

    An experimental approach to evaluate the capabilities and limitations of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for quantitative elemental speciation is presented. Two metalloproteins (superoxide dismutase, containing...

  • Screening organophosphorus nerve agent degradation products in pesticide mixtures by GC-ICPMS. Richardson, Douglas D.; Caruso, Joseph A. // Analytical & Bioanalytical Chemistry;Oct2007, Vol. 389 Issue 3, p679 

    Gas chromatography inductively coupled plasma mass spectrometry (GC-ICPMS) was utilized for the analysis of four organophosphorus nerve agent degradation products in the presence of mixtures of common organophosphorus pesticides. The first degradation products of sarin (isopropyl...

  • Double-focusing ICP-MS for the analysis of biological materials. Marchante-Gayón, Juan Manuel // Analytical & Bioanalytical Chemistry;Jun2004, Vol. 379 Issue 3, p335 

    The article focuses on double-focusing inductively coupled plasma-mass spectrometry (DF-ICP-MS), one of the most powerful analytical techniques for elemental and speciation analysis in biological materials. The advantages of ICP-MS technique are: Low detection limits, multielemental...

  • Capillary liquid separation techniques with ICP MS detection. Schaumlöffel, Dirk // Analytical & Bioanalytical Chemistry;Jun2004, Vol. 379 Issue 3, p351 

    The article presents information on the capillary liquid separation techniques with inductively coupled plasma mass spectrometer (ICP-MS) detector used in elemental speciation and metalloproteomic research. The advantage of using this technique is that high separation efficiency is combined with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics