TITLE

Advances in amino acid analysis

AUTHOR(S)
Kaspar, Hannelore; Dettmer, Katja; Gronwald, Wolfram; Oefner, Peter J.
PUB. DATE
January 2009
SOURCE
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p445
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Amino acids are important targets for metabolic profiling. For decades, amino acid analysis has been accomplished by either cation-exchange or reversed-phase liquid chromatography coupled to UV absorbance or fluorescence detection of pre-column or post-column-derivatized amino acids. Recent years have seen great progress in the development of direct-infusion or hyphenated mass spectrometry in the analysis of free amino acids in physiological fluids, because mass spectrometry not only matches optical detection in sensitivity, but also offers superior selectivity. The advent of cryo-probes has also brought NMR spectroscopy within the detection limits required for the analysis of free amino acids. But there is still room for further improvement, including expansion of the analyte spectrum, reduction of sample preparation and analysis time, automation, and synthesis of affordable isotope standards. [Figure not available: see fulltext.]
ACCESSION #
35774244

 

Related Articles

  • Ultra High-Speed Analysis of Amino Acids Using X-LC with Fluorescence Detection.  // LC-GC North America;Jun2006 Supplement, Vol. 24, p18 

    The article describes the combination of precolumn derivatization with orthophthalic aldehyde and separation on a C18 column with fluorescence detection to perform an ultra high-speed analysis of amino acids, through X-LC from Jasco Inc. The results show a reduction by a factor of more than five...

  • Sodium Azide Poisoning at a Restaurant- Dallas County, Texas, 2010. Carlo, John T.; Stringer, Joey; Jenkins, Tony; Briley, Richard T.; Klein, David; Gaul, Linda; Keene, William H.; Enmon, Larry; LeBeau, Marc A.; Waninger, Eileen M. // JAMA: Journal of the American Medical Association;9/12/2012, Vol. 308 Issue 10, p965 

    The article reports on the toxicology and epidemiologic investigations of acute onset dizziness among patrons of a restaurant of Dallas County, Texas, which was presented by the Dallas County Health and Human Services (DCHHS) in April 2010. The mass spectrometry detection of food confirmed...

  • Comparison of EMIT II, CEDIA, and DPC RIA Assays for the Detection of Lysergic Acid Diethylamide in Forensic Urine Samples. Wiegand, Russell F.; Peter, Kevin L.; Stout, Peter R.; Gehlhausen, Jay M. // Journal of Analytical Toxicology;Apr2003, Vol. 27 Issue 3, p191 

    Focuses on the comparison of three immunoassays EMIT II of Dade Behring Inc., CEDIA of Microgenics Corp., and RIA of Diagnostics Products Corp. for the detection of lysergic acid diethylamide in forensic human urine samples. Determinants of this comparison; Linearity demonstrated by these...

  • Liquid Chromatography-Tandem Mass Spectrometry for Clinical Drug Analysis of Rapamycin and for Forensic Toxicology. Wong, Steven H.Y.; Jannetto, Paul J.; Sahin, Elvan; Crockett, Jimmy; Gock, Susan B.; Jentzen, Jeffrey M.; Mason, Donald; Upham, Roger A.; Morris, Michael R.; Cooper, Donald P. // Journal of Analytical Toxicology;Apr2003, Vol. 27 Issue 3, p178 

    Assessed the feasibility of rapid analysis of an immunosuppressant named Rapamycin and for forensic toxicology by using liquid chromatography-tandem mass spectrometry (LC-MS-MS) technique. Overview of chromatographic parameters used in this analysis; Features of this technique.

  • GC-MS Method for the Detection of Citalopram in Biological Matrices. Burrows, David L.; Hagardorn, Andrea N.; Harlan, Gretel C.; Wallen, Ellen D.B.; Ferslew, Kenneth E. // Journal of Analytical Toxicology;Apr2003, Vol. 27 Issue 3, p179 

    Analyses the gas chromatography-mass spectrometry (GS-MS) method for the detection of citalopram in biological matrices. Overview of intraday and interday specimens used in this analysis; Information about a GC-MS assay for citaloparm, a selective serotonin reuptake inhibitor developed for...

  • Effects of aerosol formulation to amino acids and fatty acids contents in Haruan extract. Febriyenti; Bin Bai@Baie, Saringat; Laila, Lia // Pakistan Journal of Pharmaceutical Sciences;Jan2012, Vol. 25 Issue 1, p1 

    Haruan (Channa striatus) extract was formulated to aerosol for wound and burn treatment. Haruan extract is containing amino acids and fatty acids that important for wound healing process. The purpose of this study is to observe the effect of formulation and other excipients in the formula to...

  • The Application of 7-Chloro-4-Nitrobenzoxadiazole and 4-Fluoro-7-Nitro-2,1,3-Benzoxadiazole for The Analysis of Amines and Amino Acids Using High-Performance Liquid Chromatography. Elbashir, Abdalla A.; Suliman, Fakhr Eldin O.; Aboul-Enein, Hassan Y. // Gazi University Journal of Science;2011, Vol. 24 Issue 4, p679 

    This paper reviews analytical methods, instrumental developments and applications for derivatization of amines and amino acids with 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl) and 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) using high performance liquid chromatography (HPLC). Discussion of the...

  • Post-column labeling techniques in amino acid analysis by liquid chromatography. Rigas, Pantelis // Analytical & Bioanalytical Chemistry;Oct2013, Vol. 405 Issue 25, p7957 

    Amino acid analysis (AAA) has always presented an analytical challenge in terms of sample preparation, separation, and detection. Because of the vast number of amino acids, various separation methods have been applied taking into consideration the large differences in their chemical structures,...

  • A new approach for quantitative analysis of l-phenylalanine using a novel semi-sandwich immunometric assay. Kubota, Kazuyuki; Mizukoshi, Toshimi; Miyano, Hiroshi // Analytical & Bioanalytical Chemistry;Oct2013, Vol. 405 Issue 25, p8093 

    Here, we describe a novel method for l-phenylalanine analysis using a sandwich-type immunometric assay approach for use as a new method for amino acid analysis. To overcome difficulties of the preparation of high-affinity and selectivity monoclonal antibodies against l-phenylalanine and the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics