Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper

Slaveykova, Vera I.; Guignard, Cédric; Eybe, Tanja; Migeon, Henri-Noël; Hoffmann, Lucien
January 2009
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p583
Academic Journal
This work demonstrates the capabilities of nanoscale secondary-ion mass spectrometry, using the Cameca NanoSIMS50 ion microprobe, to detect and image the copper-ion distribution in microalgal cells exposed to nanomolar and micromolar copper concentrations. In parallel to 63Cu− secondary-ion maps, images of 12C−, 12C14N−, and 31P− secondary ions were collected and analysed. A correlation of 63Cu− secondary-ion maps with those found for 12C14N− and 31P− demonstrated the possible association of Cu with cell components rich in proteins and phosphorus. The results highlighted the potential of NanoSIMS for intracellular tracking of essential trace elements such as Cu in single cells of the microalga Chlorella kesslerii. [Figure not available: see fulltext.]


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