Single-molecule spectroscopy of fluorescent proteins

Blum, Christian; Subramaniam, Vinod
January 2009
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 2, p527
Academic Journal
The discovery and use of fluorescent proteins has revolutionized cellular biology. Despite the widespread use of visible fluorescent proteins as reporters and sensors in cellular environments the versatile photophysics of fluorescent proteins is still subject to intense research. Understanding the details of the photophysics of these reporters is essential for accurate interpretation of the biological and biochemical processes illuminated by fluorescent proteins. Some aspects of the complex photophysics of fluorescent proteins can only be observed and understood at the single-molecule level, which removes averaging inherent to ensemble studies. In this paper we review how single-molecule emission detection has helped understanding of the complex photophysics of fluorescent proteins.


Related Articles

  • Two-color fluorescence correlation spectroscopy of one chromophore: Application to the E222Q mutant of the green fluorescent protein. Jung, G.; Bra¨uchle, C.; Zumbusch, A. // Journal of Chemical Physics;2/15/2001, Vol. 114 Issue 7 

    Fluorescence correlation spectroscopy (FCS) is an important method for investigations of diffusion processes as well as of photophysical properties of fluorescing molecules. It has lately been applied in studies of the photodynamics of the green fluorescent protein (GFP). In this case FCS yields...

  • Market Profile: Fluorescence Reborn.  // Spectroscopy;Oct2001, Vol. 16 Issue 10, p12 

    Presents a market profile of fluorescence spectrophotometry. Use of fluorescence spectrophotometry; Market of traditional fluorescence spectrophotometers; Technique that kick-started the use of fluorescence; Growth in the market for fluorescence microplate readers.

  • High resolution electronic spectroscopy of 1-aminonaphthalene: S0 and S1 geometries and S1←S0 transition moment orientations. Berden, Giel; Meerts, W. Leo; Plusquellic, David F.; Fujita, Ikuo; Pratt, David W. // Journal of Chemical Physics;3/15/1996, Vol. 104 Issue 11, p3935 

    Fluorescence excitation spectroscopy at both vibrational and rotational resolution has been used to probe the changes in energy, electronic distribution, and geometry that occur when 1-aminonaphthalene (1AN) absorbs light at ∼332 nm. The 000 band of the S1←S0 transition of 1AN is red...

  • Analysis of Cellular Functions by Multipoint Fluorescence Correlation Spectroscopy. Takahashi, Y.; Sawada, R.; Ishibashi, K.; Mikuni, S.; Kinjo, M. // Current Pharmaceutical Biotechnology;Apr2005, Vol. 6 Issue 2, p159 

    The biophysical investigation of living cells is currently possible by single molecular detection methods such as fluorescence correlation spectroscopy (FCS). FCS is applied for measuring the dynamic mobility of target molecules in living cells; however, the conventional FCS systems still lack...

  • Development: Dogma overturned. Cesari, Francesca // Nature Reviews Molecular Cell Biology;Dec2008, Vol. 9 Issue 12, p924 

    The article provides information on a research about the factors in the development of the mouse embryo. It notes that an early embryo is composed of the epiblast (ectoderm) and visceral endoderm. It also discusses a research on the population of visceral endoderm cells, which were discovered as...

  • Novel antibody derivatives for proteome and high-content analysis. Schmidthals, Katrin; Helma, Jonas; Zolghadr, Kourosh; Rothbauer, Ulrich; Leonhardt, Heinrich // Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 8, p3203 

    The understanding of cellular processes and their pathophysiological alterations requires comprehensive data on the abundance, distribution, modification, and interaction of all cellular components. On the one hand, artificially introduced fluorescent fusion proteins provide information about...

  • CJZ3, a lomerizine derivative, modulates P-glycoprotein function in rat brain microvessel endothelial cells. Bian-sheng Ji; Ling He; Xiao-qu Li; Guo-qing Liu // Acta Pharmacologica Sinica;Apr2006, Vol. 27 Issue 4, p414 

    Aim: To investigate the modulatory effect of CJZ3, a lomerizine derivative, on P-glycoprotein (P-gp) function in rat brain microvessel endothelial cells (RBMEC). Methods: RBMEC were isolated and cultured in Dulbecco's modified Eagle's medium/F12 (1:1)medium, and the amount of...

  • Spectroscopy of the Ã(1B2)-X(1A1) transition of jet-cooled fluorobenzene: Laser-induced fluorescence, dispersed fluorescence, and pathological Fermi resonances. Butler, Phillip; Moss, David B.; Hongming Yin; Schmidt, Timothy W.; Kable, Scott H. // Journal of Chemical Physics;9/7/2007, Vol. 127 Issue 9, p094303 

    A detailed study of the S1(1B2)-S0(1A1) electronic transition of jet-cooled fluorobenzene has been carried out using laser-induced fluorescence and dispersed fluorescence (DF) spectroscopies. Analysis of over 40 single vibronic level DF spectra resulted in the assignment of 16 fundamental...

  • Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins. Shabahang, Shahrokh; Beltz, Richard; Gentschev, Ivaylo; Goebel, Werner; Szalay, Aladar A; Yu, Yong A.; Timiryasova, Tatyana M.; Qian Zhang // Nature Biotechnology;Mar2004, Vol. 22 Issue 3, p313 

    We have shown that bacteria injected intravenously into live animals entered and replicated in solid tumors and metastases. The tumor-specific amplification process was visualized in real time using luciferase-catalyzed luminescence and green fluorescent protein fluorescence, which revealed the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics