TITLE

Nylon-6 capillary-channeled polymer (C-CP) fibers as a hydrophobic interaction chromatography stationary phase for the separation of proteins

AUTHOR(S)
Stanelle, Rayman D.; Marcus, R. Kenneth
PUB. DATE
January 2009
SOURCE
Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 1, p273
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nylon-6 capillary-channeled polymer (C-CP) fibers are used as the stationary phase for the hydrophobic interaction chromatography (HIC) separation of a synthetic protein mixture composed of ribonuclease A, lysozyme, and holotransferrin. Nylon is a useful polymer phase for HIC as it has an alkyl backbone, while the amide functionality is hydrophilic (in fact ionic) in nature. The combination of a nonporous polymer surface of the fiber phases and high column permeability yields very efficient mass transfer characteristics, as exhibited by narrowing of peak widths with increases in mobile phase linear velocity. Retention factors and resolution were evaluated at flow rates ranging from 0.5 to 9 mL/min (linear velocities of ca. 2 to 15 mm/s) and at gradient slopes between 3.3 and 20 %B/min. Optimum resolution was achieved by employing fast flow rates (9 mL/min) and slow gradients (3 %B/min), also resulting in the highest peak capacities.
ACCESSION #
35757044

 

Related Articles

  • Effect of organoclay addition on the two-body abrasive wear characteristics of polyamide 6 nanocomposites. Srinath, G.; Gnanamoorthy, R. // Journal of Materials Science;Oct2007, Vol. 42 Issue 19, p8326 

    Polymer clay nanocomposites (PCN) exhibit improved mechanical properties due to nanolevel dispersion of clay in the polymer matrix. They also exhibit good tribological performance under dry sliding conditions. Abrasive wear behaviour of these materials would be different from dry sliding...

  • Aftertreatment of Acid Dyes on Nylon 6,6 Fibers to Improve Wetfastness and Stain Resistance. Son, Y.; Burkinshaw, S. M.; Marfell, D. J. // AATCC Review;Apr2002, Vol. 2 Issue 4, p34 

    Nylon 5,6 fibers were dyed using 1:2 pre-metallized acid dyes and non-metallized acid dyes. The acid dyeings were aftertreated using an anionic agent, a cationic agent, and fullbacktan. An attempt was made to apply these aftertreatment systems to undyed nylon fabrics used as a sole adjacent...

  • New flame-retardant polyamide fiber. Bender, Klaus; Schäch, Gunther; Rosa, Sergio // Technical Textiles / Technische Textilen;Mar2013, Vol. 56 Issue 1, pE14 

    The article discusses a study on Nexylon flame-retardant (FR) polyamide fiber and other fiber blends. Comparison shows the properties of Nylon FR versus other FR fibers. Burn test results revealed the char length of fabrics after burn test, and the flammability of different FR fibers and FR...

  • The evaluation of some flexural properties of a denture base resin reinforced with various aesthetic fibers. Doğan, Orhan Murat; Bolayir, Giray; Keskin, Selda; Dogğan, Arife; Bülent Bek // Journal of Materials Science: Materials in Medicine;Jun2008, Vol. 19 Issue 6, p2343 

    This study was performed to determine whether some flexural properties of a denture base resin material could be improved through reinforcement with five types of aesthetic fibers at 3% concentration by weight and in 2, 4, and 6 mm length. Five specimens of similar dimensions were prepared for...

  • Polyamide Fiber Reactive Chemical Treatments For Differential Dyeability. Anton, Anthony // Textile Chemist & Colorist;Feb81, Vol. 13 Issue 2, p31 

    Commercial differential acid and basic dyeable polyaniide styling yarns are made by spinning polymers to different levels of amine and/or carboxyl ends, or containing acidic or basic compounds. Similar effects can be obtained by treating polyamide yarns with end-group reactive chemicals. The use...

  • Effect of different polypropylenes and compatibilizers on the rheological, mechanical and morphological properties of nylon 6/PP blends. Agrawal, Pankaj; Oliveira, Silvia I.; Araújo, E. M.; Melo, Tomas J. A. // Journal of Materials Science;Jul2007, Vol. 42 Issue 13, p5007 

    In this work, the rheological, mechanical and morphological properties of nylon 6/polypropylene compatibilized blends were investigated. Two types of polypropylene were used. One with MFI of 40 g/10 min (PP H103) and the other with MFI of 3.5 g/10 min (PP H503). The compatibilizers used were...

  • Resin to Live.  // Salt Water Sportsman;Aug2005, Vol. 66 Issue 8, p22 

    Introduces the Sufix Siege and Elite monofilaments from Sufix North America.

  • New fiber products on Domotex 2012.  // Chemical Fibers International;Mar2012, Vol. 62 Issue 1, p6 

    The article describes briefly the nylon 66 1000- and 1300-dtex radigreen polyamide and polyolefin artificial grass yarns launched by Bergamo, Italy-based RadiciGroup at the 2012 Domotex trade show held in Hanover, Germany on January 14 to 17.

  • Chapter 1: Technical textiles market -- an overview. Byrne, Chris // Handbook of Technical Textiles;2000, p1 

    The article presents an overview of technical textile market. It states that the technical textile industry plays an important role not only in its industry itself but also to other related industries who incorporate technical textile in their industrial operations. It also discusses the supply...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics