TITLE

Structural alterations of erythrocyte membrane components induced by exhaustive exercise

AUTHOR(S)
Brzeszczynska, Joanna; Pieniazek, Anna; Gwozdzinski, Lukasz; Gwozdzinski, Krzysztof; Jegier, Anna
PUB. DATE
December 2008
SOURCE
Applied Physiology, Nutrition & Metabolism;Dec2008, Vol. 33 Issue 6, p1223
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Physical exercise was used as a model of the physiological modulator of free radical production to examine the effects of exercise-induced oxidative modifications on the physico-biochemical properties of erythrocyte membrane. The aim of our work was to investigate conformational changes of erythrocyte membrane proteins, membrane fluidity, and membrane susceptibility to disintegration. Venous blood was taken before, immediately after, and 1 h after an exhaustive incremental cycling test (30 W·min-1 ramp), performed by 11 healthy untrained males on balanced diets (mean age, 22 ± 2 years; mean body mass index, 25 ± 4.5 kg·m-2). In response to this exercise, individual maximum heart rate was 195 ± 12 beats·min-1 and maximum wattage was 292 ± 27 W. Electron paramagnetic resonance spectroscopy was used to investigate alterations in membrane proteins and membrane dynamics, and to measure production of radical species. The reducing potential of plasma (RPP) was measured using the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric-reducing ability of plasma. Exercise induced decreases in erythrocyte membrane fluidity in the polar region (p < 0.0001) and alterations in the conformational state of membrane proteins (p < 0.05). An increase in RPP was observed immediately after exercise (p < 0.001), with a further increase 1 h postexercise (p < 0.0001). Supporting measurements of lipid peroxidation showed an increase in thiobarbituric acid reactive substances immediately after exercise (p < 0.05) and at 1 h of recovery (p < 0.001); however, free radicals were not detected. Results indicate the existence of early postexercise mild oxidative stress after single-exercise performance, which induced structural changes in erythrocyte membrane components (protein aggregation) and in the membrane organization (lipids rigidization) that followed lipid peroxidation but did not lead to cellular hemolysis.
ACCESSION #
35731931

 

Related Articles

  • Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. Ajdžanović, Vladimir; Spasojević, Ivan; Filipović, Branko; Šošić-Jurjević, Branka; Sekulić, Milka; Miloševiɣ, Verica // Canadian Journal of Physiology & Pharmacology;Apr2010, Vol. 88 Issue 4, p497 

    The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can...

  • Electron paramagnetic resonance spectroscopy. Hegde, B. // Resonance: Journal of Science Education;Nov2015, Vol. 20 Issue 11, p1017 

    Recently, electron paramagnetic resonance (EPR) spectroscopy has emerged as a powerful tool to study the structure and dynamics of biological macromolecules such as proteins, protein aggregates, RNA and DNA. It is used in combination with molecular modelling to study complex systems such as...

  • Glycyrrhetic Acid Synergistically Enhances β2-Adrenergic Receptor-Gs Signaling by Changing the Location of Gαs in Lipid Rafts. Qian Shi; Yuanyuan Hou; Jie Hou; Penwei Pan; Ze Liu; Min Jiang; Jie Gao; Gang Bai; Koch, Karl-Wilhelm // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Glycyrrhetic acid (GA) exerts synergistic anti-asthmatic effects via a β2-adrenergic receptor (β2AR)-mediated pathway. Cholesterol is an important component of the structure and function of lipid rafts, which play critical roles in the β2AR-Gs-adenylate cyclase (AC)-mediated signaling...

  • How does your heart rate?  // Joe Weider's Muscle & Fitness;May94, Vol. 55 Issue 5, p21 

    Informs on how to figure target heart rate to be fit cardiovascularly. Beating capacity during workout; Formula for finding heart rate.

  • PO-RR-055-SA.  // Physical Therapy;May1997, Vol. 77 Issue 5, pS19 

    Presents an abstract of a paper entitled `Heart Rate Response During 20 Minutes of Continuous Exercise on a Large Therapeutic Roll,' by E. Gappmaier, M.E. Smith et al, presented at the Physical Therapy `97: Scientific Meeting and Exposition of the American Physical Therapy Association.

  • Exercise negates high resting heart rate.  // SportEX Health (14718154);Apr2010, Issue 24, p6 

    The article reports on a study which states that regular exercise can lessen the impact of a higher resting heart rate, which can cause death among women.

  • 30 Minutes To Bliss.  // Prevention;Nov2012, Vol. 64 Issue 11, p18 

    The article suggests for doing extra physical exercise for 30 minutes for a health heart rate.

  • Broadband (up to 10 GHz) electron-paramagnetic-resonance spectrometer: cw implementation with direct detection. Rubinson, Kenneth A. // Review of Scientific Instruments;Mar89, Vol. 60 Issue 3, p392 

    The design principles for a broadband (up to 10 GHz) electron-paramagnetic-resonance (EPR) spectrometer are presented. The upper frequency limit is determined by the losses in the coaxial cables. The sample is inserted along the central conductor at the end of a short-circuited coaxial line that...

  • EPR study of iron-doped MFI zeolite and silicalite catalysts: effect of treatments after synthesis. Ferretti, Anna M.; Forni, Lucio; Oliva, Cesare; Ponti, Alessandro // Research on Chemical Intermediates;2002, Vol. 28 Issue 2/3, p101 

    The systematic study of iron-doped MFI zeolite and MFI silicalite by X- and Q-band EPR spectroscopy is reported in this paper. Both samples, which contain Fe(III) ions in the MFI lattice, underwent three sequential treatments�calcination, steaming, and washing with sodium dithionite...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics