TITLE

Non-Linear Effects in the Formation of DNA Damage in Medaka Fish Fibroblast Cells Caused by Combined Action of Cadmium and Ionizing Radiation

AUTHOR(S)
Grygoryev, Dmytro; Moskalenko, Oleksandr; Zimbrick, John D.
PUB. DATE
July 2008
SOURCE
Dose-Response;2008, Vol. 6 Issue 3, p283
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Ionizing radiation-induced formation of genomic DNA damage can be modulated by nearby chemical species such as heavy metal ions, which can lead to non-linear dose response. To investigate this phenomenon, we studied cell survival and formation of 8-hydroxyguanine (8-OHG) base modifications and double strand breaks (DSB) caused by combined action of cadmium (Cd) and gamma radiation in cultured medaka fish (Oryzias latipes) fibroblast cells. Our data show that the introduction of Cd leads to a significant decrease in the fraction of surviving cells and to increased sensitivity of cells to ionizing radiation (IR). Cd also appears to cause non-linear increases in radiation-induced yields of 8-OHG and DSB as dose-yield plots of these lesions exhibit non-linear S-shaped curves with a sharp increase in the yields of lesions in the 10-20 μM range of Cd concentrations. The combined action of ionizing radiation and Cd leads to increased DNA damage formation compared to the effects of the individual stressors. These results are consistent with a hypothesis that the presence of Cd modulates the efficiency of DNA repair systems thus causing increases in radiation-induced DNA damage formation and decreases in cell survival.
ACCESSION #
35560592

 

Related Articles

  • Repair of DNA Double-Strand Breaks is Not Modulated by Low-Dose Gamma Radiation in C57BL/6J Mice. Blimkie, Melinda S. J.; Fung, Luke C. W.; Petoukhov, Eugenia S.; Girard, Cyrielle; Klokov, Dmitry // Radiation Research;May2014, Vol. 181 Issue 5, p548 

    In this study, we sought to determine whether low-dose ionizing radiation, previously shown to induce a systemic adaptive response in C57BL/6J mice, is capable of enhancing the rate of DNA double-strand break repair. Repair capacity was determined by measuring γ-H2AX levels in splenic and...

  • Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage. Essers, Jeroen; Houtsmuller, Adriaan B.; van Veelen, Lieneke; Paulusma, Coen; Nigg, Alex L.; Pastink, Albert; Vermeulen, Wim; Hoeijmakers, Jan H.J.; Kanaar, Roland // EMBO Journal;4/15/2002, Vol. 21 Issue 8, p2030 

    Recombination between homologous DNA molecules is essential for the proper maintenance and duplication of the genome, and for the repair of exogenously induced DNA damage such as double-strand breaks. Homologous recombination requires the RAD52 group proteins, including Rad51, Rad52 and Rad54....

  • Molecular and phenotypic responses of Japanese medaka ( Oryzias latipes) early life stages to environmental concentrations of cadmium in sediment. Barjhoux, Iris; Gonzalez, Patrice; Baudrimont, Magalie; Cachot, Jérôme // Environmental Science & Pollution Research;Sep2016, Vol. 23 Issue 18, p17969 

    Japanese medaka embryos were exposed to environmental concentrations of cadmium (Cd) to investigate adverse and adaptive responses in fish early life stages. Embryos were exposed during their whole development by static sediment-contact to environmental Cd concentrations (2 and 20 μg/g dry...

  • Plasma induced DNA damage: Comparison with the effects of ionizing radiation. Lazović, S.; Maletić, D.; Leskovac, A.; Filipović, J.; Puač, N.; Malović, G.; Joksić, G.; Petrović, Z. Lj. // Applied Physics Letters;9/22/2014, Vol. 105 Issue 12, p1 

    We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand...

  • Dose-Dependent Reactive Species Accumulation and Preferential Double-Strand Breaks Repair are Featured in the γ-ray Response in Medicago truncatula Cells. Donà, Mattia; Ventura, Lorenzo; Balestrazzi, Alma; Buttafava, Armando; Carbonera, Daniela; Confalonieri, Massimo; Giraffa, Giorgio; Macovei, Anca // Plant Molecular Biology Reporter;Feb2014, Vol. 32 Issue 1, p129 

    In plants, there are issues related to the effects of gamma (γ)-rays that are still poorly explored, particularly as concerns the biological response to irradiation delivered at a low dose rate. In the present work, the effects of exposure to increasing γ-ray total doses (6, 12, 25, and 50...

  • DNA Damage Caused by Chronic Transgenerational Exposure to Low Dose Gamma Radiation in Medaka Fish (Oryzias latipes). Grygoryev, D.; Moskalenko, O.; Hinton, T. G.; Zimbrick, J. D. // Radiation Research;Sep2013, Vol. 180 Issue 3, p235 

    The effect of transgenerational exposure to low dose rate (2.4 and 21 mGy/day) gamma irradiation on the yield of DNA double-strand breaks and oxidized guanine (8-hydroxygua-nine) has been studied in the muscle and liver tissue of a model organism, the Japanese medaka fish. We found the level of...

  • Chemical Radioprotection and Radiosensitization of Mammalian Cells Growing in Vitro. Chapman, J. D.; Reuvers, A. P.; Borsa, J.; Greenstock, C. L. // Radiation Research;Aug2012, Vol. 178 Issue 2, pAV214 

    The radiosensitizing and radioprotective effects of various compounds have been characterized in Chinese hamster fibroblasts growing in vitro and in a model chemical system utilizing DNA as target. The contribution to the lethal action of ionizing radiation in mammalian cells from the indirect...

  • "ATR activation in response to ionizing radiation: still ATM territory.". Cuadrado, Myriam; Martinez-Pastor, Barbara; Fernandez-Capetillo, Oscar // Cell Division;2006, Vol. 1, p7 

    Unrepaired DNA double-strand breaks (DSBs) are a major cause for genomic instability. Therefore, upon detection of a DSB a rapid response must be assembled to coordinate the proper repair/signaling of the lesion or the elimination of cells with unsustainable amounts of DNA damage. Three members...

  • DNA repair: a key mechanism stabilizing the genome. Lavrik, O. // Biochemistry (00062979);Jan2011, Vol. 76 Issue 1, p3 

    No abstract available.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics