TITLE

Implementation of terbium-sensitized luminescence in sequential-injection analysis for automatic analysis of orbifloxacin

AUTHOR(S)
Llorent-Martínez, E.; Ortega-Barrales, P.; Molina-Díaz, A.; Ruiz-Medina, A.
PUB. DATE
December 2008
SOURCE
Analytical & Bioanalytical Chemistry;Dec2008, Vol. 392 Issue 7/8, p1397
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Orbifloxacin (ORBI) is a third-generation fluoroquinolone developed exclusively for use in veterinary medicine, mainly in companion animals. This antimicrobial agent has bactericidal activity against numerous Gram-negative and Gram-positive bacteria. A few chromatographic methods for its analysis have been described in the scientific literature. Here, coupling of sequential-injection analysis and solid-phase spectroscopy is described in order to develop, for the first time, a terbium-sensitized luminescent optosensor for analysis of ORBI. The cationic resin Sephadex-CM C-25 was used as solid support and measurements were made at 275/545 nm. The system had a linear dynamic range of 10–150 ng mL−1, with a detection limit of 3.3 ng mL−1 and an R.S.D. below 3% ( n = 10). The analyte was satisfactorily determined in veterinary drugs and dog and horse urine.
ACCESSION #
35354222

 

Related Articles

  • Evaluación de la Actividad Antimicrobiana de Extractos de Propóleos de la Huasteca Potosina (México). Carrillo, María L.; Castillo, Laura N.; Mauricio, Rosalba // Información Tecnológica;2011, Vol. 22 Issue 5, p21 

    The antimicrobial activity of ethanolic and aqueous extracts of propolis in the Huasteca Potosina, in Mexico was evaluated. Strains of Gram negative: Escherichia coli, Salmonella typhi and Klebsiella pneumoniae, and Gram positive microorganisms: Staphylococcus aureus, Staphylococcus epidermidis...

  • Enhanced Bioactivity of Ag/ZnO Nanorods-A Comparative Antibacterial Study. Shah, A. H.; Manikandan, E.; Ahmed, M. Basheer; Ganesan, V. // Journal of Nanomedicine & Nanotechnology;2013, Vol. 4 Issue 3, p1 

    Background: In the present work, Ag/ZnO nanorods were synthesized in order to investigate bacterial sensitivity against Gram positive and Gram negative bacteria, comparatively by using well diffusion method. Materials and methods: Nanoparticles were synthesized by the Sol-Gel method, using...

  • An Overview of Fluoroquinolones. Somasundaram, Sulochana; Manivannan, Kiruthika // Annual Review & Research in Biology;Jul-Sep2013, Vol. 3 Issue 3, p296 

    The fluoroquinolones are broad-spectrum bactericidal agents inhibiting DNA synthesis. They are active against gram positive and gram negative organisms like P. aeruginosa, Mycoplasma, Chlamydia, Staphylococci and a few of the Streptococci. They are useful in the treatment of pneumonia, urinary...

  • Enhanced Recovery and Identification of a Tryptamine-Related Antibiotic Produced by Intrasporangium N8 from KwaZulu-Natal, South Africa. Okudoh, Vincent I.; Wallis, Frederick M. // Tropical Journal of Pharmaceutical Research;Oct2012, Vol. 11 Issue 5, p729 

    Purpose: To isolate and identify an antibiotic produced by a soil bacterium, Intrasporangium strain N8, with antibacterial activity against both Gram-positive and Gram-negative bacteria. Methods: Fermentation followed by extraction using a three-solvent system (petroleum ether, acetone and ethyl...

  • Studies on Antibacterial, Anthelmintic and Antioxidant activities of a Macrolichen Parmotrema pseudotinctorum (des. Abb.) Hale (Parmeliaceae) from Bhadra wildlife sanctuary, Karnataka. Praveen Kumar, S. V.; Prashith Kekuda, T. R.; Vinayaka, K. S.; Sudharshan, S. J.; Mallikarjun, N.; Swathi, D. // International Journal of PharmTech Research;Apr2010, Vol. 2 Issue 2, p1207 

    The present study was carried to determine the antibacterial, anthelmintic and antioxidant activity of a macrolichen Parmotrema pseudotinctorum (des. Abb.) Hale (Parmeliaceae) collected from forest area of Bhadra wildlife sanctuary. The extract exhibited marked antibacterial activity. The...

  • The Lyophilization Process Maintains the Chemical and Biological Characteristics of Royal Jelly. Piacezzi Nascimento, Andresa; Roveroni Moraes, Larissa Ariana; Ursoli Ferreira, Nathália; de Padua Moreno, Gabriela; Mangolini Uahib, Fernanda Grassi; Aparecida Barizon, Edna; Aparecida Berretta, Andresa // Evidence-based Complementary & Alternative Medicine (eCAM);2015, p1 

    The alternative use of natural products, like royal jelly (RJ), may be an important tool for the treatment of infections caused by antibiotic-resistant bacteria. RJ presents a large number of bioactive substances, including antimicrobial compounds. In this study, we carried out the chemical...

  • Antibacterial activity of environmentally `green' alternative products tested in standard... Bauer, Janice M.; Beronio, Carol A. // Journal of Environmental Health;Mar1995, Vol. 57 Issue 7, p13 

    Examines the ability of `green' products to kill or eliminate representative Gram positive and Gram negative bacteria from nonporous surfaces. Antimicrobial activity of the products; Alternative products; Mechanical removal of bacteria from surfaces.

  • You Asked For It. Hunter, Beatrice Trum // Consumers' Research Magazine;Dec2001, Vol. 84 Issue 12, p9 

    Presents answers to questions regarding food topics. Information on Ritalin; Information on gram-positive and gram-negative bacteria; How food is freeze-dried; Use of a commercial wash for cleaning fruits and vegetables.

  • Differential sensitivity of Staphylococcus epidermidis and Escherichia coli to lysozyme. Benathen, Isaiah A.; McKenzie, La Shon // Journal of Biological Education (Society of Biology);Winter98, Vol. 33 Issue 1, p49 

    Demonstrates a standard dilution and confluent plate procedure that shows the enhanced sensitivity of gram-positive bacteria to lysozyme, and the moderate sensitivity of gram-negative bacteria to this enzyme. Preparation of lysozyme; Consequences of lysozyme action.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics