TITLE

Micromechanical observation of the kinetics of biomolecular interactions

AUTHOR(S)
Taeyun Kwon; Kilho Eom; Jinsung Park; Dae Sung Yoon; Hong Lim Lee; Tae Song Kim
PUB. DATE
October 2008
SOURCE
Applied Physics Letters;10/27/2008, Vol. 93 Issue 17, p173901
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.
ACCESSION #
35279029

 

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