TITLE

Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens

AUTHOR(S)
Sainsus, N.; Cattori, V.; Lepadatu, C.; Hofmann-Lehmann, R.
PUB. DATE
December 2008
SOURCE
European Journal of Clinical Microbiology & Infectious Diseases;Dec2008, Vol. 27 Issue 12, p1209
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The goal of this study was to develop a liquid culture medium for the rapid isolation, cultivation, identification and subsequent antibiotics susceptibility testing of Helicobacter pylori directly from biopsy specimens. Five liquid media were tested: Ham’s F-12, Brucella broth, tryptic soybroth, brain heart infusion broth and Mueller-Hinton broth. After optimisation of the medium, it was applied in order to investigate biopsy samples from 150 patients with gastro-duodenal disorders and compared with traditional culture methods, microscopy and an H. pylori-specific TaqMan real-time polymerase chain reaction (PCR). The most reliable and rapid growth of H. pylori, even at a small inoculum size, was obtained in Ham’s F-12 medium with 5% horse serum. The developed system allowed the primary isolation of H. pylori in clinical samples and provided 87% sensitivity and 100% specificity.
ACCESSION #
35261484

 

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