Isolation and characterization of microsatellite loci in Pedicularis verticillata L. using PCR-based isolation of microsatellite arrays (PIMA)

Tsurng-Juhn Huang; Yi-Yen Chen; Yen-Ping Li; Cheng-Yu Hung; Tzen-Yuh Chiang; Chang-Hung Chou
October 2008
Conservation Genetics;Oct2008, Vol. 9 Issue 5, p1389
Academic Journal
Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (HE) and observed (H0) heterozygosity was 0.62609-0.89662 and 0-0.95652, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure, which, in turn, can provide information for establishing conservation strategy for these endangered species.


Related Articles

  • Microsatellites for Next-Generation Ecologists: A Post-Sequencing Bioinformatics Pipeline. Fernandez-Silva, Iria; Whitney, Jonathan; Wainwright, Benjamin; Andrews, Kimberly R.; Ylitalo-Ward, Heather; Bowen, Brian W.; Toonen, Robert J.; Goetze, Erica; Karl, Stephen A. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Microsatellites are the markers of choice for a variety of population genetic studies. The recent advent of next-generation pyrosequencing has drastically accelerated microsatellite locus discovery by providing a greater amount of DNA sequencing reads at lower costs compared to other techniques....

  • Characterization of target nuclear DNA from faeces reduces technical issues associated with the assumptions of low-quality and quantity template. Ball, Mark C.; Pither, Richard; Manseau, Micheline; Clark, Jeff; Petersen, Stephen D.; Kingston, Steve; Morrill, Natasha; Wilson, Paul // Conservation Genetics;Jun2007, Vol. 8 Issue 3, p577 

    Faecal material has increasingly become an important non-invasive source of DNA for wildlife population genetics. However, DNA from faecal sources can have issues associated with quantity (low-template and/or low target-to-total DNA ratio) and quality (degradation and/or low DNA-to-inhibitor...

  • Development of soybean aphid genomic SSR markers using next generation sequencing. Jun, Tae-Hwan; Michel, Andrew P.; Mian, M.A. Rouf; Gustafson, Perry // Genome;May2011, Vol. 54 Issue 5, p360 

    Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid ( Matsumura) has become the most damaging...

  • Microsatellite primed-PCR to select molecular markers for Tuber species. Amicucci, Antonella; Guidi, Chiara; Potenza, Lucia; Stocchi, Vilberto // Biotechnology Letters;Feb2002, Vol. 24 Issue 4, p263 

    The direct microsatellite-primed PCR and the RAMPO techniques were applied to detect inter-specific polymorphisms in Tuber species and to select species specific fragments. A T. borchii marker was identified and specific primers were selected.

  • Development of Microsatellite Markers in Pungtungia herzi Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia. Young-Eun Yun; Jeong-Nam Yu; Sang Ki Kim; Ui Wook Hwang; Kwak, Myounghai // International Journal of Molecular Sciences;Oct2013, Vol. 14 Issue 10, p19923 

    Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain...

  • BatchPrimer3: A high throughput web application for PCR and sequencing primer design. You, Frank M.; Huo, Naxin; Yong Qiang Gu; Ming-cheng Luo; Yaqin Ma; Hane, Dave; Lazo, Gerard R.; Dvorak, Jan; Anderson, Olin D. // BMC Bioinformatics;2008, Vol. 9, Special section p1 

    Background: Microsatellite (simple sequence repeat -- SSR) and single nucleotide polymorphism (SNP) markers are two types of important genetic markers useful in genetic mapping and genotyping. Often, large-scale genomic research projects require high-throughput computer-assisted primer design....

  • Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages. Tailleux, Ludovic; Waddell, Simon J.; Pelizzola, Mattia; Mortellaro, Alessandra; Withers, Michael; Tanne, Antoine; Castagnoli, Paola Ricciardi; Gicquel, Brigitte; Stoker, Neil G.; Butcher, Philip D.; Foti, Maria; Neyrolles, Olivier // PLoS ONE;2008, Vol. 3 Issue 1, p1 

    Background. Transcriptional profiling using microarrays provides a unique opportunity to decipher host pathogen cross-talk on the global level. Here, for the first time, we have been able to investigate gene expression changes in both Mycobacterium tuberculosis, a major human pathogen, and its...

  • Comparison of ISSR polymorphism among cattle breeds. Stolpovsky, Yu.; Ahani Azari, M.; Evsukov, A.; Kol, N.; Ruzina, M.; Voronkova, V.; Sulimova, G. // Russian Journal of Genetics;Feb2011, Vol. 47 Issue 2, p189 

    Polymorphism analysis of DNA fragments flanked by (AG)C and (GA)C inverted dinucleotide microsatellite repeats in 766 animals of 19 cattle breeds and one breeding type revealed 66 fragments, of which 64 were polymorphic. The breeds proved to differ in the frequency and presence or absence of...

  • PCR multiplexing for maximising genetic analyses with limited DNA samples: an example in the collared flycatcher, Ficedula albicollis. Karaiskou, Nikoletta; Primme, Craig // Annales Zoologici Fennici;2008, Vol. 45 Issue 6, p478 

    This article discusses a study that developed a series of microsatellite multiplexes using a recently released commercial multiplex buffer in order to demonstrate the potential usefulness of polymerase chain reaction multiplexing in a non-model organism. The study focused on the collared...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics