High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota

Zoetendal, E. G.; Rajiliá-Stojanovié, M.; de Vos, W. M.
November 2008
Gut;Nov2008, Vol. 57 Issue 11, p1605
Academic Journal
The human gastrointestinal (61) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the 61 tract microbiota has been derived from the application of small subunit ribosomal ANA (SSU rRNA)-based technologies. These not only provided a phylogenetic framework of the GI tract microbiota, the majority of which has not yet been cultured, but also advanced insights into the impact of host and environmental factors on the microbiota community structure and dynamics. In addition, it emerged that 61 tract microbial communities are host and 61 tract location-specific. This complicates establishing relevant links between the host's health and the presence or abundance of specific microbial populations and argues for the implementation of novel high-throughput technologies in studying the diversity and functionality of the GI tract microbiota. Here, we focus on the recent developments and applications of phylogenetic microarrays based on SSU rRNA sequences and metagenomics approaches exploiting rapid sequencing technologies in unravelling the secrets of our 61 tract microbiota.


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