Histological And Histochemical Studies After Partial Hepatoectomy In Liver And Spleen Of Rats

Motaal, Abdel
June 2008
Egyptian Journal of Hospital Medicine;Jun2008, Vol. 31, p165
Academic Journal
Introduction :- The aim of this investigation is to study the effect of partial hepatoectomy on the histology and histochemistry of liver and spleen of male rats. Material And Methods:- Twenty four rats were used in the present study and were divided into four groups"six rats each".group(I) and classified into four groups: Negative control group (I). 6 rats and group (II) hepatoectomized rats were given sub. Cut 0.1 ml vitamin K 3 days consequently before surgical hepatectomy was performed. Histopathological examination of liver and spleen and frozen sections for alkaline phosphatase demonstration were undertaken in the 1st day, 7th day and 15th day following the surgical operation. Results:- The histopathological results were manifested in hepatoectomized rats as congested portal vein, vacuolation and pyknosis in liver cells, fibrosis inflammatory cells infiltration around hepatic vein and increasing kupffer cells. In splenic tissue the histopathological changes were in the form of ill defined pulps with marked congestion and necrotic nuclei. After 15th days of operation marked recovery in both white and red pulps were recorded. The histochemical examination of alkaline phosphatase revealed marked attenuation after 1th and 7th days of operation in both liver and spleen of hepatoectomized rats followed by regeneration after 15th day of operation in comparison to the control group.


Related Articles

  • Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues. Kudo, Lili C.; Vi, Nancy; Ma, Zhongcai; Fields, Tony; Avliyakulov, Nuraly K.; Haykinson, Michael J.; Bragin, Anatol; Karsten, Stanislav L. // PLoS ONE;Jul2012, Vol. 7 Issue 7, p1 

    We developed a novel, highly accurate, capillary based vacuum-assisted microdissection device CTAS - Cell and Tissue Acquisition System, for efficient isolation of enriched cell populations from live and freshly frozen tissues, which can be successfully used in a variety of molecular studies,...

  • THE EFFECT OF PROLONGED INGESTION OF SYNTHETIC VITAMIN K IN RATS. HATTON, E. H.; DODDS, A.; HODGE, H. C.; FOSDICK, L. S. // Journal of Dental Research;Dec1945, Vol. 24 Issue 6, p283 

    This article discusses the results of a research study conducted to determine the effects of the incorporation of different levels of a synthetic vitamin K, menadione, into the diets of rats. In rats fed the highest percentage of menadione (.8 percent), the researchers found a marked loss in...

  • Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells.  // Lipids in Health & Disease;2011, Vol. 10 Issue 1, p158 

    The article cites a research study that analyzes the function of vitamin K in the testis and analyzed its role in steroidogenesis. It is said that vitamin K is vital for the posttranslational modification of various Gla proteins. The function of vitamin K in several organs, including the testis,...

  • Frozen Section Examination of Liver, Gallbladder, and Pancreas. Lechago, Juan // Archives of Pathology & Laboratory Medicine;Dec2005, Vol. 129 Issue 12, p1610 

    Context.-Frozen section of the liver is a comparatively frequent request that most often applies to a relatively limited number of situations. The only indication for frozen tissue examination of a gallbladder with any frequency is the presence of a polypoid mucosal lesion or a suspicious...

  • Protective effect of some vitamins against the toxic action of ethanol on liver regeneration induced by partial hepatectomy in rats. Columbano, Amedeo; Mars, Wendy Michelle; Ramírez-Farías, Carlett; Madrigal-Santillán, Eduardo; Gutiérrez-Salinas, José; Rodríguez-Sánchez, Nidia; Martínez-Cruz, Maricela; Valle-Jones, Ilse; Gramlich-Martínez, Ingrid; Hernández-Ceruelos, Alejandra; Morales-González, José A.; Neuberger, James // World Journal of Gastroenterology;2/14/2008, Vol. 14 Issue 6, p899 

    AIM: To investigate the effects of vitamins (A, C and E) on liver injury induced by ethanol administration during liver regeneration in rats. METHODS: Male Wistar rats subjected to 70% partial hepatectomy were divided into five groups (groups 1-5). During the experiment, animals of Group 1 drank...

  • The strategy of combined ischemia preconditioning and salvianolic acid-B pretreatment to prevent hepatic ischemia-reperfusion injury in rats. Rui Kong; Yue Gao; Bei Sun; Hua Chen; Gang Wang; Xiuyun Wang; Hong Zhu; Shangha Pan; Dongbo Xue; Hongchi Jiang; Kong, Rui; Gao, Yue; Sun, Bei; Chen, Hua; Wang, Gang; Wang, Xiuyun; Zhu, Hong; Pan, Shangha; Xue, Dongbo; Jiang, Hongchi // Digestive Diseases & Sciences;Dec2009, Vol. 54 Issue 12, p2568 

    Background: Ischemia-reperfusion injury (IRI) is a serious complication of liver surgery, especially for extended hepatectomy and liver transplantation. The aim of this study was to evaluate the protective effect of combined ischemic preconditioning (IPC) and salvianolic acid-B...

  • Butyrate Protects Rat Liver against Total Hepatic Ischemia Reperfusion Injury with Bowel Congestion. Liu, Bin; Qian, Jianmin; Wang, Qingbao; Wang, Fangrui; Ma, Zhenyu; Qiao, Yingli // PLoS ONE;Aug2014, Vol. 9 Issue 8, p1 

    Hepatic ischemia/reperfusion (I/R) injury is an unavoidable consequence of major liver surgery, especially in liver transplantation with bowel congestion, during which endotoxemia is often evident. The inflammatory response aggravated by endotoxin after I/R contributes to liver dysfunction and...

  • Effect of Solder Fumes on Liver Serum Markers and Hepatic Vascular Elements in Rats. Arab, Mohammad Reza; Mirzaei, Ramezan; Aval, Feridoon Sargolzaei; Nakhaei, Ali Reza; Karimi, Mehrbod; Mashhadi, Rezvaneh // Yakhteh Medical Journal;Spring2010, Vol. 12 Issue 1, p33 

    Objective: This study was performed to determine the effects of solder fume on liver serum markers and vascular elements in rats Materials and Methods: A total number of 48 rats were randomly divided into experimental (n=30) and control (n=18) groups. Based on exposure time, each group was...

  • HEPATOPROTECTIVE ACTIVITY OF NELSONIA CANESCENS (LAM.) SPRENG ON ACUTE HEPATOTOXICITY INDUCED BY PARACETAMOL. Dasgupta, Bedabati; Kalita, Jogen Chandra; Chowdhury, Arudyuti; Kotoky, Jibon // International Journal of Pharmacy & Pharmaceutical Sciences;Jan2012 Supplement, Vol. 4, p107 

    The methanol extract (MLE) of the plant Nelsonia canescens (Lam.) Spreng at different doses (150, 300 and 500 mg/kg, b.w.) was tested for its efficacy against paracetamol induced acute hepatic damage in Wistar rats. The different groups of rats were administered with paracetamol (2 gm/kg, p.o.)....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics