TITLE

The structure of mollusc larval shells formed in the presence of the chitin synthase inhibitor Nikkomycin Z

AUTHOR(S)
Schönitzer, Veronika; Weiss, Ingrid M.
PUB. DATE
January 2007
SOURCE
BMC Structural Biology;2007, Vol. 7, p71
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Chitin self-assembly provides a dynamic extracellular biomineralization interface. The insoluble matrix of larval shells of the marine bivalve mollusc Mytilus galloprovincialis consists of chitinous material that is distributed and structured in relation to characteristic shell features. Mollusc shell chitin is synthesized via a complex transmembrane chitin synthase with an intracellular myosin motor domain. Results: Enzymatic mollusc chitin synthesis was investigated in vivo by using the small-molecule drug NikkomycinZ, a structural analogue to the sugar donor substrate UDP-N-acetyl-D-glucosamine (UDP-GlcNAc). The impact on mollusc shell formation was analyzed by binocular microscopy, polarized light video microscopy in vivo, and scanning electron microscopy data obtained from shell material formed in the presence of NikkomycinZ. The partial inhibition of chitin synthesis in vivo during larval development by NikkomycinZ (5 µM - 10 µM) dramatically alters the structure and thus the functionality of the larval shell at various growth fronts, such as the bivalve hinge and the shell's edges. Conclusion: Provided that NikkomycinZ mainly affects chitin synthesis in molluscs, the presented data suggest that the mollusc chitin synthase fulfils an important enzymatic role in the coordinated formation of larval bivalve shells. It can be speculated that chitin synthesis bears the potential to contribute via signal transduction pathways to the implementation of hierarchical patterns into chitin mineral-composites such as prismatic, nacre, and crossed-lamellar shell types.
ACCESSION #
35113280

 

Related Articles

  • Aggregation of Partially Unfolded Myosin Subfragment-1 into Spherical Oligomers with Amyloid-Like Dye-Binding Properties. Komatsu, Hideyuki; Shinotani, Nami; Kimori, Yoshitaka; Tokuoka, Jun-ichiro; Kaseda, Kuniyoshi; Nakagawa, Hiroyuki; Kodama, Takao // Journal of Biochemistry;Jun2006, Vol. 139 Issue 6, p989 

    Proteolytic myosin subfragment 1 (S1) is known to be partially unfolded in its 50-kDa subdomain by mild heat treatment at 35°C [Burke et al. (1987) Biochemistry 26, 1492–1496]. Here, we report that this partial unfolding is accompanied by aggregation of S1 protein. Characteristics of...

  • Glycolytic activity of enzyme preparation from the red king crab ( Paralithodes camtschaticus) hepatopancreas. Rysakova, K.; Novikov, V.; Mukhin, V.; Serafimchik, E. // Applied Biochemistry & Microbiology;May2008, Vol. 44 Issue 3, p251 

    Enzyme preparation exhibiting glycolytic activity yielding chitooligosaccharides along with N-acetyl- D-glucosamine was obtained from the red king crab ( Paralithodes camtschaticus) hepatopancreas. The results of the analysis confirmed the presence of endo- and exochitinase activities in the...

  • Protein Crystallography in Drug Discovery. Canduri, Fernanda; de Azevedo Jr., Walter Filgueira // Current Drug Targets;Dec2008, Vol. 9 Issue 12, p1048 

    Protein crystallography is the main technique used to obtain three-dimensional information for binary complexes involving protein and drugs. Once a protein target has its three-dimensional structure elucidated, the next natural step is the solving of the structure complexed either with its...

  • The structure of Mycobacteria 2C-methyl-D-erythritol-2, 4-cyclodiphosphate synthase, an essential enzyme, provides a platform for drug discovery. Buetow, Lori; Brown, Amanda C.; Parish, Tanya; Hunter, William N. // BMC Structural Biology;2007, Vol. 7, p68 

    Background: The prevalence of tuberculosis, the prolonged and expensive treatment that this disease requires and an increase in drug resistance indicate an urgent need for new treatments. The 1-deoxy-D-xylulose 5-phosphate pathway of isoprenoid precursor biosynthesis is an attractive...

  • Characterization and role of a metalloprotease induced by chitin in Serratia sp. KCK. Hyun-Soo Kim; Golyshin, Peter N.; Timmis, Kenneth N. // Journal of Industrial Microbiology & Biotechnology;Nov2007, Vol. 34 Issue 11, p715 

    A metalloprotease induced by chitin in a new chitinolytic bacterium Serratia sp. Strain KCK was purified and characterized. Compared with other Serratia enzymes, it exhibited a rather broad pH activity range (pH 5.0–8.0), and thermostability. The cognate ORF, mpr, was cloned and...

  • Inhibitors of Steroidal Cytochrome P450 Enzymes as Targets for Drug Development. Baston, Eckhard; Leroux, Fr�d�ric R. // Recent Patents on Anti-Cancer Drug Discovery;Jan2007, Vol. 2 Issue 1, p31 

    Cytochrome P450's are enzymes which catalyze a large number of biological reactions, for example hydroxylation, N-, O-, S- dealkylation, epoxidation or desamination. Their substrates include fatty acids, steroids or prostaglandins. In addition, a high number of various xenobiotics are...

  • Privileged Structures: A Useful Concept for the Rational Design of New Lead Drug Candidates. Duarte, Carolina D.; Barreiro, Eliezer J.; Fraga, Carlos A. M. // Mini Reviews in Medicinal Chemistry;Nov2007, Vol. 7 Issue 11, p1108 

    Privileged structures are defined as molecular frameworks which are able of providing useful ligands for more than one type of receptor or enzyme target by judicious structural modifications. In the present work, we describe some examples and applications of the usefulness of the privileged...

  • Glutathione mediated regulation of oligomeric structure and functional activity of Plasmodium falciparum glutathione S-transferase. Tripathi, Timir; Rahlfs, Stefan; Becker, Katja; Bhakuni, Vinod // BMC Structural Biology;2007, Vol. 7, p67 

    Background: In contrast to many other organisms, the malarial parasite Plasmodium falciparum possesses only one typical glutathione S-transferase. This enzyme, PfGST, cannot be assigned to any of the known GST classes and represents a most interesting target for antimalarial drug development....

  • Protective effect of Solanum nigrum fruit extract on the functional status of liver and kidney against ethanol induced toxicity. Arulmozhi, V.; Krishnaveni, M.; Mirunalini, S. // Journal of Biochemical Technology;2012, Vol. 3 Issue 4, p339 

    The trend in using natural products has increased and the active plant extracts are frequently screened for new drug discoveries. The present study was aimed to investigate the effect of Solanum nigrum fruit extract (SNFEt) on ethanol induced toxicity in rats. Male albino wistar rats were...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics