Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by Polymerase Chain Reaction in patients with sterile pyuria

Nassar, Fadel A.; Abu-Elamreen, Farid H.; Shubair, Mohammad E.; Sharif, Fadel A.
June 2008
Advances in Medical Sciences (De Gruyter Open);2008, Vol. 53 Issue 1, p80
Academic Journal
Purpose: Chlamydia trachomatis and Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. To determine the occurrence of Chlamydia traehomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum in patients with sterile pyuria. Material/Methods: Sterile pyuria urine samples collected during the period from February 2006 to April 2007 were tested by polymerase chain reaction (PCR) for the presence of C. trachomatis, M. hominis, M. genitalium, and U. urealyticum using specific primers for each species. A total of 200 sterile pyuria samples selected from about 2400 urine samples attending the genitourinary clinic at Al-Shifa hospital, Gaza, during the period February 2006 to April 2007 and were analyzed for routine urine examination and cultured on MacConkey agar, blood agar, and sabouraud agar to detect the presence of bacteria and Candida. The 200 samples (96 male, 104 female; aged ≥ 18 years) containing more than 10 leukocytes / HPF and negative for culture (showing no significant growth after 24 hr) were tested by PCR for C. trachomatis and M. hominis, M. genitalium, and U. urealyticum. Results: C. trachomatis was detected in 20 samples (10%), U urealyticum in 10 samples (5%), M. hominis in 6 samples (3%) and M. genitalium in 2 samples (1%). The difference in occurrence of C. trachomatis was statistically insignificant between males and females (P=0.509), but it was significant (P=0.008) for U. urealyticum. M. hominis was detected only in samples collected from female patients. On the other hand, M. genitalium was detected only in men. Conclusion: PCR testing of sterile pyuria showed a significant number of C. trachomatis, Mycoplasma, and Ureaplasma infections. Consequently, PCR is recommended for the detection of those microorganisms in the urine samples of sterile pyuria patients.


Related Articles

  • Associations between Mycoplasma genitalium, Chlamydia trachomatis and pelvic inflammatory disease. Simms I; Eastick K; Mallinson H; Thomas K; Gokhale R; Hay P; Herring A; Rogers P A // Journal of Clinical Pathology;Aug2003, Vol. 56 Issue 8, p616 

    OBJECTIVE: To evaluate the association between Mycoplasma genitalium, Chlamydia trachomatis, and pelvic inflammatory disease (PID) METHODS: A case-control methodology was used. Swab eluates were processed using the QIAamp DNA mini kit. Polymerase chain reaction (PCR) for M genitalium was carried...

  • Detection of Chlamydia trachomatis and Mycoplasma genitalium in semen samples of infertile men using multiplex PCR. Sadrpour, Parisa; Bahador, Abbas; Asgari, Soheila; Bagheri, Rezvan; Chamani-Tabriz, Leili // Tehran University Medical Journal;Jan2013, Vol. 70 Issue 10, p623 

    Background: Chlamydia trachomatis is the most common bacterial sexually transmitted infection in the world, but the effect of this infection on male fertility is still controversial. Despite reports of interaction between Mycoplasma genitalium and sperm, this pathogen in semen samples of...

  • A comparison of self-taken vulvovaginal and cervical samples for the diagnosis of Chlamydia trachomatis infection by polymerase chain reaction. Keane, F. E. A.; Bendall, Richard; Saulsbury, N.; Haddon, Lisa // International Journal of STD & AIDS;Feb2007, Vol. 18 Issue 2, p98 

    Implementation of the National Chlamydia Screening Programme in Cornwall commenced in April 2003. Initially, women in community venues were screened using urine samples. However, many of these urine samples were inhibitory to polymerase chain reaction (PCR), resulting in a high recall rate for...

  • Potential public health benefits from testing with Chlamydia trachomatis PCR technique on first void urine in men. Norén, Lena; Von Krogh, Geo; Bondesson, Lena; Nohlgård, Christina; Grillner, Lena // Acta Dermato-Venereologica;4/20/98, Vol. 78 Issue 1, p63 

    Urine samples from 467 men living in the Stockholm area were tested with the polymerase chain reaction (PCR), Roche Amplicor, and with an enzyme-linked immunosorbent assay, Syva MicroTrak EIA, for detection of Chlamydia trachomatis. The predictive value of urine versus urethral samples was...

  • Is new variant Chlamydia trachomatis present in England and Wales? Alexander, S.; Ison, C. // Sexually Transmitted Infections;Feb2008, Vol. 84 Issue 1, p29 

    Background: A new variant C trachomatis (nvCT) strain has been recently isolated in Sweden, which has a 377 bp deletion in a portion of the plasmid that is the target area for some C trachomatis nucleic acid amplification tests (NAATs). Consequently, these platforms generate false negative...

  • Conjunctivitis Associated with Mycoplasma genitalium Infection. Björnelius, Eva; Jensen, Jörgen Skov; Lidbrink, Peter // Clinical Infectious Diseases;10/1/2004, Vol. 39 Issue 7, pe67 

    Conjunctival and first-void urine specimens from a patient with unilateral conjunctivitis and urethritis were found to be positive for Mycoplasma genitalium but negative for Chlamydia trachomatis, by polymerase chain reaction analysis. Identical M. genitalium DNA sequences were found in both...

  • Mycoplasma genitalium in non-gonococcal urethritis--a study in Swedish male STD patients. Björnelius, Eva; Lidbrink, Peter; Jensen, Jørgen Skov; Björnelius, E; Lidbrink, P; Jensen, J S // International Journal of STD & AIDS;May2000, Vol. 11 Issue 5, p292 

    Urethral swab specimens obtained from 101 men attending an STD clinic were examined for the presence of Mycoplasma genitalium by polymerase chain reaction (PCR) amplification. Fifty patients had non-gonococcal urethritis (NGU), and 51 patients were included as controls without urethritis. M....

  • PCR is Useful in Detecting Chlamydia Trachomatis.  // Fertility Weekly;9/24/2007, p9 

    The article reports on the study conducted by researchers regarding the function of polymerase chain reaction (PCR) in detecting chlamydia trachomatis (CT) in Turkey. Researchers Melih A. Guven and colleagues have conducted a prospective investigation of the rate of CT, mycoplasma hominis and...

  • Mycoplasma genitalium: prevalence, clinical significance, and transmission. Anagrius, C.; Loré, B.; Jensen, J. S. // Sexually Transmitted Infections;Dec2005, Vol. 81 Issue 6, p458 

    Objectives: To study the prevalence, symptoms and signs of Mycoplasma genitalium and Chlamydia trachomatis infections in STD clinic attendees and in partners of M genitalium infected patients. Methods: M genitalium and C trachomatis were detected by polymerase chain reaction from urethral and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics