Structural disorder promotes assembly of protein complexes

Hegyi, Hedi; Schad, Eva; Tompa, Peter
January 2007
BMC Structural Biology;2007, Vol. 7, p65
Academic Journal
Background: The idea that the assembly of protein complexes is linked with protein disorder has been inferred from a few large complexes, such as the viral capsid or bacterial flagellar system, only. The relationship, which suggests that larger complexes have more disorder, has never been systematically tested. The recent high-throughput analyses of protein-protein interactions and protein complexes in the cell generated data that enable to address this issue by bioinformatic means. Results: In this work we predicted structural disorder for both E. coli and S. cerevisiae, and correlated it with the size of complexes. Using IUPred to predict the disorder for each complex, we found a statistically significant correlation between disorder and the number of proteins assembled into complexes. The distribution of disorder has a median value of 10% in yeast for complexes of 2-4 components (6% in E. coli), but 18% for complexes in the size range of 11-100 proteins (12% in E. coli). The level of disorder as assessed for regions longer than 30 consecutive disordered residues shows an even stronger division between small and large complexes (median values about 4% for complexes of 2-4 components, but 12% for complexes of 11-100 components in yeast). The predicted correlation is also supported by experimental evidence, by observing the structural disorder in protein components of complexes that can be found in the Protein Data Bank (median values 1. 5% for complexes of 2-4 components, and 9.6% for complexes of 11-100 components in yeast). Further analysis shows that this correlation is not directly linked with the increased disorder in hub proteins, but reflects a genuine systemic property of the proteins that make up the complexes. Conclusion: Overall, it is suggested and discussed that the assembly of protein-protein complexes is enabled and probably promoted by protein disorder.


Related Articles

  • Phylogenetic background of E. coli isolated from asymptomatic pregnant women from Kolkata, India. Mukherjee, Mandira; Koley, Snehashis; Mukherjee, SandipKumar; Basu, Shreya; Ghosh, Biplab; Chakraborty, Somajita // Journal of Infection in Developing Countries;Jul2015, Vol. 9 Issue 7, p720 

    Introduction: Asymptomatic bacteriuria (ABU) in pregnancy generates medical complications. E. coli is the common etiologic agent responsible for ABU-associated infections. This study aimed to identify the phylogenetic background and drug resistance in asymptomatic E. coli from a pregnant...

  • Enteroaggregative Escherichia coli Have Evolved Independently as Distinct Complexes within the E. coli Population with Varying Ability to Cause Disease. Chattaway, Marie Anne; Jenkins, Claire; Rajendram, Dunstan; Cravioto, Alejandro; Talukder, Kaisar Ali; Dallman, Tim; Underwood, Anthony; Platt, Steve; Okeke, Iruka N.; Wain, John // PLoS ONE;Nov2014, Vol. 9 Issue 11, p1 

    Enteroaggregative E. coli (EAEC) is an established diarrhoeagenic pathotype. The association with virulence gene content and ability to cause disease has been studied but little is known about the population structure of EAEC and how this pathotype evolved. Analysis by Multi Locus Sequence...

  • Phenotypic Microarrays Suggest Escherichia coli ST131 Is Not a Metabolically Distinct Lineage of Extra-Intestinal Pathogenic E. coli. Alqasim, Abdulaziz; Emes, Richard; Clark, Gemma; Newcombe, Jane; La Ragione, Roberto; McNally, Alan // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli...

  • Epidemiological, Clinical and Bacteriological Studies on Bacterial Lamb Enteritis at Behera Province, Egypt. Nasr, Mohammad; Nabil, M. Bakeer; Hammouda, Hassan A.; Alaa, A. Omar // Alexandria Journal for Veterinary Sciences;2014, Vol. 43 Issue 1, p8 

    Lamb enteritis is economically important problem because several agents may be involved in the etiology of it. This study was conducted to throw light on bacterial enteritis in some sheep flocks in Behera province. From 1200 examined lambs, 650 showed enteritis (54.16 %). Enteritis rate were...

  • An exploratory study of dog park visits as a risk factor for exposure to drug-resistant extra-intestinal pathogenic E. coli (ExPEC). Ahmed, Lubna N.; Price, Lance B.; Graham, Jay P. // BMC Research Notes;2015, Vol. 8 Issue 1, p1 

    Background: Extraintestinal pathogenic E. coli (ExPEC) are common causative agents of urinary tract infections in humans. Dogs have been found to harbor ExPEC. This study tested stool samples from dogs (n = 16), the shoes of dog park visitors (n = 16) and the shoes of controls (n = 16) for...

  • Genotypic and phenotypic features of enteropathogenic Escherichia coli isolated in industrialized and developing countries. Santona, Simona; Diaz, Nicia; Fiori, Pier Luigi; Francisco, Moises; Sidat, Mohsin; Cappuccinelli, Piero; Rappelli, Paola // Journal of Infection in Developing Countries;Mar2013, Vol. 7 Issue 3, p214 

    Introduction: Typical EPEC are considered a leading cause of diarrhoea in developing countries, while atypical EPEC have been isolated more frequently in developed areas. The actual geographic distribution of the two EPEC subgroups is controversial, since data can be highly influenced by...

  • Genomic and Phenomic Study of Mammary Pathogenic Escherichia coli. Blum, Shlomo E.; Heller, Elimelech D.; Sela, Shlomo; Elad, Daniel; Edery, Nir; Leitner, Gabriel // PLoS ONE;9/1/2015, Vol. 10 Issue 9, p1 

    Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E....

  • Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector. Taghipour, N.; Bandepour, M.; Pazoki, R.; Haghighi, A.; Nazari^Pouya, M. R.; Kazemi, B. // Iranian Journal of Parasitology;2009, Vol. 4 Issue 4, p1 

    Background: Echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus Echinococcus, family Taeniidae. We aimed to subclone antigen B gene in pQE-30 plasmid, its expression, and purification. Methods: We subcloned HI gene...

  • Nuclease-Assisted Suppression of Human DNA Background in Sepsis. Song, Yajing; Giske, Christian G.; Gille-Johnson, Patrik; Emanuelsson, Olof; Lundeberg, Joakim; Gyarmati, Peter // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    Sepsis is a severe medical condition characterized by a systemic inflammatory response of the body caused by pathogenic microorganisms in the bloodstream. Blood or plasma is typically used for diagnosis, both containing large amount of human DNA, greatly exceeding the DNA of microbial origin. In...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics