TITLE

Genomic conservation of cattle microsatellite loci in wild gaur (Bos gaurus) and current genetic status of this species in Vietnam

AUTHOR(S)
Trung Thanh Nguyen; Sem Genini; Linh Chi Bui; Voegeli, Peter; Stranzinger, Gerald; Renard, Jean-Paul; Maillard, Jean-Charles; Bui Xuan Nguyen
PUB. DATE
January 2007
SOURCE
BMC Genetics;2007, Vol. 8, p77
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The wild gaur (Bos gaurus) is an endangered wild cattle species. In Vietnam, the total number of wild gaurs is estimated at a maximum of 500 individuals. Inbreeding and genetic drift are current relevant threats to this small population size. Therefore, information about the genetic status of the Vietnamese wild gaur population is essential to develop strategies for conservation and effective long-term management for this species. In the present study, we performed cross-species amplification of 130 bovine microsatellite markers, in order to evaluate the applicability and conservation of cattle microsatellite loci in the wild gaur genome. The genetic diversity of Vietnamese wild gaur was also investigated, based on data collected from the 117 successfully amplified loci. Results: One hundred-thirty cattle microsatellite markers were tested on a panel of 11 animals. Efficient amplifications were observed for 117 markers (90%) with a total of 264 alleles, and of these, 68 (58.1%) gave polymorphic band patterns. The number of alleles per locus among the polymorphic markers ranged from two to six. Thirteen loci (BM1314, BM2304, BM6017, BMC2228, BMS332, BMS911, CSSM023, ETH123, HAUT14, HEL11, HEL5, ILSTS005 and INRA189) distributed on nine different cattle chromosomes failed to amplify wild gaur genomic DNA. Three cattle Ychromosome specific microsatellite markers (INRA124, INRA126 and BM861) were also highly specific in wild gaur, only displaying an amplification product in the males. Genotype data collected from the 117 successfully amplified microsatellites were used to assess the genetic diversity of this species in Vietnam. Polymorphic Information Content (PIC) values varied between 0.083 and 0.767 with a mean of 0.252 while observed heterozygosities (Ho) ranged from 0.091 to 0.909 (mean of 0.269). Nei's unbiased mean heterozygosity and the mean allele number across loci were 0.298 and 2.2, respectively. Conclusion: Extensive conservation of cattle microsatellite loci in the wild gaur genome, as shown by our results, indicated a high applicability of bovine microsatellites for genetic characterization and population genetic studies of this species. Moreover, the low genetic diversity observed in Vietnamese wild gaur further underlines the necessity of specific strategies and appropriate management plans to preserve this endangered species from extinction.
ACCESSION #
34971124

 

Related Articles

  • Identification of Quantitative Trait Loci Affecting Cattle Temperament. BeatrizGutiérez-Gil; Ball, Nia; Burton, Deborah; Haskell, Marie; Williams, John L.; Wiener, Pamela // Journal of Heredity;Nov/Dec2008, Vol. 99 Issue 6, p629 

    in addition to its potential contribution to improving animal welfare, the study of the genetics of cattle behavior may provide more general insights into the genetic control of such complex traits. We carried out a genome scan in a Holstein x Charolais cross cattle population to identify...

  • Complex Microsatellite Dynamics in the Myostatin Gene Within Ruminants. Tellgren-Roth, Åsa; Kolesov, Grigory; Sifuentes-Rincón, Ana m.; Liberles, David a. // Journal of Molecular Evolution;Mar2008, Vol. 66 Issue 3, p258 

    A microsatellite has previously been identified in myostatin in cattle. Sequencing of this region from other artiodactyls coupled with phylogenetic analysis has been used to uncover the potential origins of the microsatellite event, which appears either to have been born twice or to have been...

  • A whole genome scan for differences in recombination rates among three Bos taurus breeds. Thomsen, Hauke; Reinsch, Norbert; Xu, Ningying; Bennewitz, Jörn; Looft, Christian; Grupe, Sven; Kühn, Christa; Brockmann, Gudrun A.; Schwerin, Manfred; Leyhe-Horn, Birgit; Hiendleder, Stefan; Erhardt, Georg; Medjugorac, Ivica; Russ, Ingolp; Förster, Martin; Brenig, Bertram; Reinhardt, Fritz; Reents, Reinhard; Blümel, Jürgen; Averdunk, Gottfried // Mammalian Genome;Sep2001, Vol. 12 Issue 9, p724 

    Twenty paternal half-sib families of a granddaughter design were genotyped for 265 genetic markers, most of them microsatellites. These were 16 Holstein families, 3 Simmental families, and 1 Brown Swiss family. The number of sires per breed was 872, 170, and 32, respectively. Two-point...

  • A Microsatellite Variability Screen for Positive Selection Associated With the "Out of Africa" Habitat Expansion of Drosophila melanogaster. Kauer, M.O.; Dieringer, D.; Schlötterer, C. // Genetics;Nov2003, Vol. 165 Issue 3, p1137 

    We report a "hitchhiking mapping" study in D. melanogaster, which searches for genomic regions with reduced variability. The study's aim was to identify selective sweeps associated with the "out of Africa" habitat expansion. We scanned 103 microsatellites on chromosome 3 and 102 microsatellites...

  • A Type I and Type II microsatellite linkage map of Rainbow trout (Oncorhynchus mykiss) with presumptive coverage of all chromosome arms. Guyomard, Rene; Mauger, Stephane; Tabet-Canale, Kamila; Martineau, Sylvain; Genet, Carine; Krieg, Francine; Quillet, Edwige // BMC Genomics;2006, Vol. 7, p1 

    Background: The development of large genomic resources has become a prerequisite to elucidate the wide-scale evolution of genomes and the molecular basis of complex traits. Linkage maps represent a first level of integration and utilization of such resources and the primary framework for...

  • Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa. Tuskan, Gerald A.; Gunter, Lee E.; Yang, Zamin K.; TongMing Yin; Sewell, Mitchell M.; DiFazio, Stephen P. // Canadian Journal of Forest Research;Jan2004, Vol. 34 Issue 1, p85 

    Microsatellites or simple sequence repeats (SSRs) are highly polymorphic, codominant markers that have great value for the construction of genetic maps, comparative mapping, population genetic surveys, and paternity analyses. Here, we report the development and testing of a set of SSR markers...

  • Mapping of the bovine spinal muscular atrophy locus to Chromosome 24. Medugorac, Ivica; Kemter, Juliane; Russ, Ingolf; Pietrowski, Detlef; Nüske, Stefan; Reichenbach, Horst-Dieter; Schmahl, Wolfgang; Förster, Martin // Mammalian Genome;Jun2003, Vol. 14 Issue 6, p383 

    A hereditary form of spinal muscular atrophy (SMA) caused by an autosomal recessive gene has been reported for American Brown-Swiss cattle and in advanced backcrosses between American Brown-Swiss and many European brown cattle breeds. Bovine SMA (bovSMA) bears remarkable resemblance to the human...

  • Genetic diversity studies of Kherigarh cattle based on microsatellite markers. Pandey, A. K.; Sharma, Rekha; Singh, Yatender; Prakash, B. B.; Ahlawat, S. P. S. // Journal of Genetics;Aug2006, Vol. 85 Issue 2, p117 

    We report a genetic diversity study of Kherigarh cattle, a utility draught-purpose breed of India, currently declining at a startling rate, by use of microsatellite markers recommended by the Food and Agriculture Organization. Microsatellite genotypes were derived, and allelic and genotypic...

  • Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Blair, M. W.; Pedraza, F.; Buendia, H. F.; Gaitán-Solís, E.; Beebe, S. E.; Gepts, P.; Tohme, J. // Theoretical & Applied Genetics;Nov2003, Vol. 107 Issue 8, p1362 

    A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics