TITLE

Fourier transform infrared spectroscopy as a tool to study structural properties of cytochromes P450 (CYPs)

AUTHOR(S)
Jung, Christiane
PUB. DATE
November 2008
SOURCE
Analytical & Bioanalytical Chemistry;Nov2008, Vol. 392 Issue 6, p1031
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cytochrome P450 proteins (CYPs) are a big class of heme proteins which are involved in various metabolic processes of living organisms. CYPs are the terminal catalytically active components of monooxygenase systems where the substrate binds and is hydroxylated. In order to be functionally competent, the protein structures of CYPs possess specific properties that must be explored in order to understand structure–function relationships and mechanistic aspects. Fourier transform infrared spectroscopy (FTIR) is one tool that is used to study these structural properties. The application of FTIR spectroscopy to the secondary structures of CYP proteins, protein unfolding, protein–protein interactions and the structure and dynamics of the CYP heme pocket is reviewed. A comparison with other thiolate heme proteins (nitric oxide synthase and chloroperoxidase) is also included. [Figure not available: see fulltext.]
ACCESSION #
34940182

 

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