TITLE

2′F-Arabinonucleic acids (2′F-ANA) – History, properties, and new frontiers

AUTHOR(S)
Watts, Jonathan K.; Damha, Masad J.
PUB. DATE
July 2008
SOURCE
Canadian Journal of Chemistry;Jul2008, Vol. 86 Issue 7, p641
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The development of arabinonucleosides and oligoarabinonucleotides is described, focusing especially on 2′-deoxy-2′-fluoroarabinonucleosides (araF-N) and -oligonucleotides (2'F-ANA). In addition to their chemical and enzymatic synthesis, we discuss various properties of 2′F-ANA: hydrolytic stability (to nucleases, acids, and bases), binding affinity to complementary strands, structure and conformation, and optimization of RNase H activity. We also discuss the use of 2′F-ANA in gene-silencing approaches (antisense, siRNA), and in the stabilization of higher-order structures (such as triplexes and quadruplexes) including aptamers. Finally, we examine several other oligonucleotide derivatives based on 2′F-ANA and look ahead to the future of 2′-fluoroarabinonucleosides and -oligonucleotides.
ACCESSION #
34899762

 

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