TITLE

Protective effect of estrogen on apoptosis in a cell culture model of Parkinson's disease

AUTHOR(S)
Xue-Li Li; Wei-Dong Cheng; Juan Li; Xian-Ling Guo; Cun-Ju Guo; Xian-Hui Meng; Sheng-Gang Sun; Le-Xin Wang
PUB. DATE
October 2008
SOURCE
Clinical & Investigative Medicine;Oct2008, Vol. 31 Issue 5, pE258
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objectives: The protective effect of estrogen on the neurons in Parkinson's disease (PD) is unclear. The present study aimed to investigate the effect of estrogen on the apoptosis and dopaminergic function on a cultured cell model of PD. Methods: The PD model was established by addition of 1-methyl-4-phenylpyridinium (MPP+) to PC12 cell culture. Estrogen was added to cell groups with MPP+ (Estrogen+MPP+), and without MPP+ (Estrogen only group). Cell viability, content of tyrosine hydroxylase (TH), apoptosis ratio, expression of apoptosis-suppression protein Bcl-x and apoptosis-acceleration protein IL-1 beta converting enzyme (ICE) were measured. Results: Cell viability in the Estrogen+MPP+ group was similar to the control group but was higher than in the MPP+ group (P<0.05). The apoptosis ratios in the Estrogen+MPP+ group (33.6%), and the control group (31.3%), were also similar, but it was lower than in the MPP+ group (63.5%, P<0.05). Concentrations of Bcl-x were higher in the Estrogen+MPP+ group, whereas ICE concentrations were lower than in the MPP+ group (P<0.05). Conclusions: Estrogen suppresses apoptosis and improves cell viability in MPP+ induced injuries in the PC12 cells. The beneficial effects of estrogen on the PD model are due to the suppression of pro-apoptotic protein ICE, and stimulation of anti-apoptotic protein Bcl-x.
ACCESSION #
34879930

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics