Human Metapneumovirus Reinfection among Children in Thailand Determined by ELISA Using Purified Soluble Fusion Protein

Pavlin, Julie A.; Hickey, Andrew C.; Ulbrandt, Nancy; Yee-Peng Chan; Endy, Timothy P.; Boukhvalova, Marina S.; Chunsuttiwat, Supamit; Nisalak, Ananda; Libraty, Daniel H.; Green, Sharone; Rothman, Alan L.; Ennis, Francis A.; Jarman, Richard; Gibbons, Robert V.; Broder, Christopher C.
September 2008
Journal of Infectious Diseases;9/15/2008, Vol. 198 Issue 6, p836
Academic Journal
Background. Human metapneumovirus (hMPV) is a newly discovered paramyxovirus that causes acute respiratory illness. Despite apparent near-universal exposure during early childhood, immunity is transient. Methods. An indirect screening enzyme-linked immunosorbent assay using a recombinant soluble fusion (F) glycoprotein derived from hMPV was used to test for anti-F IgG in 1380 pairs of acute- and convalescent-stage serum samples collected from children in Kamphaeng Phet, Thailand. Results. Of the 1380 serum sample pairs tested, 1376 (99.7%) showed evidence of prior infection with hMPV. Sixty-six paired specimens demonstrated a ⩾4-fold rise in titer, for an overall reinfection rate of 4.9%. Two children demonstrated evidence of an initial infection. Forty-eight of the 68 new infections or reinfections occurred in 2000, accounting for 13.2% of all nonflaviviral febrile illnesses in the study population in that year. Of 68 positive cases, 85.3% complained of cough and 66.2% complained of rhinorrhea, compared with 61.4% and 49.0% of negative cases, respectively (P < .01). All positive samples were also tested for an increase in titer of antibodies to respiratory syncytial virus F, and 27% exhibited a ⩾4-fold rise. Conclusion. These results demonstrate that hMPV reinfections cause illness at a rate equal to that seen for initial infections. hMPV may have a more significant impact in older children than previously realized and may be the cause of significant outbreaks in this population.


Related Articles

  • Evaluation of Confounders in Toxoplasmosis Indirect Fluorescent Antibody Assay. Saraei, M.; Shojaee, S.; Esmaeli, A. R.; Jahani- Hashemi, H.; Keshavarz, H. // Iranian Journal of Parasitology;2010, Vol. 5 Issue 4, p55 

    Background: The IFA test is one of the most usual methods for detecting anti-Toxoplasma antibodies, although it has not any unique standardization. It seems that the microscopic judgment of results is an important confounder in IFA test. Therefore, we conducted the present study to clarify the...

  • Immunogenicity of a Heptavalent Pneumococcal Conjugate Vaccine in Apache and Navajo Indian,... Miernyk, Karen M.; Parkinson, Alan J.; Rudolph, Karen M.; Petersen, Kenneth M.; Bulkow, Lisa R.; Greenberg, David P.; Ward, Joel I.; Brenneman, George; Reid, Ray; Santosham, Mathuram // Clinical Infectious Diseases;7/1/2000, Vol. 31 Issue 1, p34 

    Determines the immunogenicity of a heptavalent pneumococcal conjugate vaccine in Alaska Native, Apache and Navajo Indian and non-Native American children in the United States. Measurement of serotype-specific immunoglobulin G levels by a standardized enzyme-linked immunosorbent assay;...

  • Evaluation of Cerebrospinal Fluid for the Presence of Anticardiolipin Antibodies (aCL) in NP-SLE Patients. J&ecedil;dryka-Góral, A.; Z&acedil;bek, J.; Wojciechowska, B.; Zaborski, J.; Chwalińska-Sadowska, H.; Cz&lslash;onkowska, A. // Clinical Rheumatology;Jul2000, Vol. 19 Issue 4, p306 

    : Many neurological or psychiatric manifestations of SLE (NP-SLE) are related to the presence of anticardiolipin antibodies (aCL) in the patient’s sera. The aim of this study was to evaluate the presence of aCL in cerebrospinal fluid (CSF) in SLE patients with NP features. Fifteen SLE...

  • Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti-β2-Glycoprotein I Antibodies Isolation. Artenjak, Andrej; Leonardi, Adrijana; Križaj, Igor; Ambrožiž, Aleš; Sodin-Semrl, Snezna; Božič, Borut; Čučnik, Saša // Journal of Immunology Research;2014, p1 

    Patient biological material for isolation of β2-glycoprotein I (?2GPI) and high avidity IgG anti-β2-glycoprotein I antibodies (HAv anti-β2GPI) dictates its full utilization. The aim of our study was to evaluate/improve procedures for isolation of unnicked β2GPI and HAv aβ2GPI to...

  • Tamm-Horsfall glycoprotein binds IgG with high affinity. Rhodes, Diana C. J.; Hinsman, Edward J.; Rhodes, James A. // Kidney International;Nov1993, Vol. 44 Issue 5, p1014 

    Tamm-Horsfall protein (THP). a monomeric glycoprotein (Mr 80 to 100 kDa), is produced by the mammalian kidney's thick ascending limb of Henle cells and excreted into the urine. The function of THP is uncertain. Here we report that a high molecular weight contaminant in sheep THP (sTHP)...

  • Quantitative assays for anti-aquaporin-4 antibody with subclass analysis in neuromyelitis optica. Isobe, Noriko; Yonekawa, Tomomi; Matsushita, Takuya; Kawano, Yuji; Masaki, Katsuhisa; Yoshimura, Satoshi; Fichna, Jakub; Chen, Shu; Furmaniak, Jadwiga; Smith, Bernard Rees; Kira, Jun-ichi // Multiple Sclerosis Journal;Nov2012, Vol. 18 Issue 11, p1541 

    The article presents research that examines the quantitative assays for anti-aquaporin-4 (anti-AQP4) antibody with neuromyelitis optica subclass analysis. The study shows no association between anti-AQP4 antibody immunoglobulin G (IgG) subclasses or antibody positivity and clinical parameters...

  • Estandarizaci�n en Colombia de una prueba ELISA para la evaluaci�n de los niveles s�ricos de anticuerpos IgG contra diez serotipos de Streptococcus pneumoniae. Leal-Esteban, Luc�a Carolina; Rojas, Jessica Lineth; Jaimes, Andrea Lizeth; Montoya, Juan David; Montoya, Nilton Edu; Leiva, Lily; Trujillo-Vargas, Claudia Milena // Biom�dica: Revista del Instituto Nacional de Salud;mar2012, Vol. 32 Issue 1, p92 

    Introduction. Streptococcus pneumoniae is a major cause of morbi-mortality in early childhood and elderly. However, a test to measure the antibody responses after specific vaccination is not available in Colombia. Objective. An immunoenzymatic test was standardized for the measurement of serum...

  • Determination of ochratoxin A by polyclonal antibodies based sensitive time-resolved fluoroimmunoassay. Biao Huang; Wenyi Tao; Jin Shi; Luhong Tang; Jian Jin // Archives of Toxicology;Aug2006, Vol. 80 Issue 8, p481 

    Using indirect competitive time-resolved fluoroimmunoassay (TRFIA), a rapid, highly selective and extremely sensitive method has been established for the determination of ochratoxin A (OA). Tests can be performed in a 96-well microplate using the toxin-specific polyclonal antibodies, obtained...

  • Equine cestodosis: a sero-epidemiological study of Anoplocephala perfoliata infection in Ethiopia. Getachew, A.; Innocent, G.; Proudman, C.; Trawford, A.; Feseha, G.; Reid, S.; Faith, B.; Love, S. // Veterinary Research Communications;Apr2012, Vol. 36 Issue 2, p93 

    A 12/13 kDa antigen, tapeworm ELISA test, developed for use in horses, was used to detect parasite-specific serum antibody, IgG(T), in the serum of donkeys. In a pilot study the 12/13 kDa antigen was tested and proved to detect the antibody, IgG(T), in donkey sera. Blood samples from 797...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics