TITLE

The Role of Hypoxia in the Differentiation of P19 Embryonal Carcinoma Cells into Dopaminergic Neurons

AUTHOR(S)
Li-Ying Wu; Yue Wang; Bing Jin; Tong Zhao; Hai-Tao Wu; Yan Wu; Ling-Ling Zhu
PUB. DATE
October 2008
SOURCE
Neurochemical Research;Oct2008, Vol. 33 Issue 10, p2118
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Nervous system development at early stage is in hypoxic environment. Very little is known about the role of hypoxia in neuronal development. P19 embryonal carcinoma (EC) cells are a widely used model for studying early neuronal development. In this study we investigated the roles of hypoxia in differentiation of dopaminergic neurons derived from P19 EC cells. Results demonstrate that hypoxia increases the percentage of differentiated neurons, especially neurons of dopaminergic phenotype. To investigate the potential mechanism involved in hypoxia promoted differentiation of dopaminergic neurons, we measured the expression of hypoxia-inducible factor 1α (HIF-1α), based on its characteristic response to hypoxia. The result shows that HIF-1α mRNA level in P19 EC cells increases after hypoxia treatment. It is known that HIF-1α regulates the expression of tyrosine hydroxylase (TH) gene through binding to its promoter. Therefore, we propose that the underlying mechanism for hypoxia promoted differentiation of dopaminergic neurons was mediated by HIF-1α up-regulation under hypoxia.
ACCESSION #
34305505

 

Related Articles

  • IL-32γ inhibits cancer cell growth through inactivation of NF-κB and STAT3 signals. Oh, J H; Cho, M-C; Kim, J-H; Lee, S Y; Kim, H J; Park, E S; Ban, J O; Kang, J-W; Lee, D-H; Shim, J-H; Han, S B; Moon, D C; Park, Y H; Yu, D-Y; Kim, J-M; Kim, S H; Yoon, D-Y; Hong, J T // Oncogene;7/28/2011, Vol. 30 Issue 30, p3345 

    Several studies have shown physiological functions of interleukin (IL)-32, a novel cytokine. However, the role of IL-32 in cancer development has not been reported. In this study, we showed that IL-32γ inhibited tumor growth in IL-32γ-overexpressing transgenic mice inoculated with melanoma...

  • Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3. Yang, Jianhua; Yan, Chunxiao; Zheng, Wei; Chen, Xuejun // Archives of Gynecology & Obstetrics;Jan2012, Vol. 285 Issue 1, p239 

    Aims: The purpose of this study is to investigate the association between proliferation inhibition of cisplatin and aquaporin 5 (AQP5) expression and its regulation in ovarian carcinoma cell CAOV3. Methods: Cell growth rate was measured by MTT after CAOV3 cells were incubated with cisplatin or...

  • The Role of Receptor for Advanced Glycation End Products (RAGE) in the Proliferation of Hepatocellular Carcinoma. Yaser, Al-Madhagi; Yan Huang; Rong-Rong Zhou; Guan-Sheng Hu; Mei-Fang Xiao; Zhe-Bing Huang; Chao-Jun Duan; Wei Tian; Dao-Lin Tang; Xue-Gong Fan // International Journal of Molecular Sciences;May2012, Vol. 13 Issue 5, p5982 

    The receptor for advanced glycation end products (RAGE) is oncogenic and overexpressed in human cancers, but its role in hepatocellular carcinoma remains unclear. Here we demonstrated that RAGE is overexpressed in primary hepatocellular carcinoma (PHC) compared to adjacent para-neoplastic liver...

  • Epigenetic changes and nuclear factor-κB activation, but not microRNA-224, downregulate Raf-1 kinase inhibitor protein in triple-negative breast cancer SUM 159 cells. LABBOZZETTA, MANUELA; POMA, PAOLA; VIVONA, NICOLETTA; GULINO, ALESSANDRO; D'ALESSANDRO, NATALE; NOTARBARTOLO, MONICA // Oncology Letters;Dec2015, Vol. 10 Issue 6, p3807 

    Raf-1 kinase inhibitor protein (RKIP) is a tumor suppressor and metastasis inhibitor, which enhances drug-induced apoptosis of cancer cells. Downregulation of RKIP may be significant in the biology of highly aggressive and drug-resistant tumors, for example triple-negative breast cancers...

  • Nanosecond Pulsed Electric Field Inhibits Cancer Growth Followed by Alteration in Expressions of NF-κB and Wnt/β-Catenin Signaling Molecules. Ren, Zhigang; Chen, Xinhua; Cui, Guangying; Yin, Shengyong; Chen, Luyan; Jiang, Jianwen; Hu, Zhenhua; Xie, Haiyang; Zheng, Shusen; Zhou, Lin // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Cancer remains a leading cause of death worldwide and total number of cases globally is increasing. Novel treatment strategies are therefore desperately required for radical treatment of cancers and long survival of patients. A new technology using high pulsed electric field has emerged from...

  • Celastrol Suppresses Tumor Cell Growth through Targeting an AR-ERG-NF-κB Pathway in TMPRSS2/ERG Fusion Gene Expressing Prostate Cancer. Shao, Longjiang; Zhou, Zhansong; Cai, Yi; Castro, Patricia; Dakhov, Olga; Shi, Ping; Bai, Yaoxia; Ji, Huixiang; Shen, Wenhao; Wang, Jianghua // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    The TMPRSS2/ERG (T/E) fusion gene is present in the majority of all prostate cancers (PCa). We have shown previously that NF-kB signaling is highly activated in these T/E fusion expressing cells via phosphorylation of NF-kB p65 Ser536 (p536). We therefore hypothesize that targeting NF-kB...

  • Relationship between NFKB1 −94 insertion/deletion ATTG polymorphism and susceptibility of cervical squamous cell carcinoma risk. Zhou, B.; Qie, M.; Wang, Y.; Yan, L.; Zhang, Z.; Liang, A.; Wang, T.; Wang, X.; Song, Y.; Zhang, L. // Annals of Oncology;Mar2010, Vol. 21 Issue 3, p506 

    Background: A very high expression of nuclear factor-kappa B protein (nuclear p50, encoded by NFKB1) in high-grade squamous intraepithelial lesion and invasive cancers has been observed. The aim of this study was to determine whether the functional NFKB1 −94 insertion/deletion ATTG...

  • Research Highlights.  // Nature Medicine;Jan2008, Vol. 14 Issue 1, p23 

    The article offers news briefs related to medicine. A study published in "Genes & Development" showed that the transcription factor NF-kB plays a significant role in the aging of people. Stem researchers reported that the transcription factor Foxa2 can sustain dopamine neurons. A report in the...

  • NF-kappaB Inhibits Expression of the alpha1(I) Collagen Gene. Rippe, Richard A.; Schrum, Laura W.; Stefanovic, Branko; Solis-Herruzo, Jose A.; Brenner, David A. // DNA & Cell Biology;Oct99, Vol. 18 Issue 10, p751 

    Fibrosis results from an increase in the synthesis and deposition of type I collagen. Fibrosis is frequently associated with inflammation, which is accompanied by increased levels of tumor necrosis factor-alpha (TNF alpha) and activation of the transcription factor NF-kappaB. However, several...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics