Green Tea Polyphenol Epigallocatechin-3-Gallate Suppresses Collagen Production and Proliferation in Keloid Fibroblasts via Inhibition of the STAT3-Signaling Pathway

Park, Gyuman; Yoon, Byung Sun; Moon, Jai-Hee; Kim, Bona; Jun, Eun Kyoung; Oh, Sejong; Kim, Hyunggee; Song, Hea Joon; Noh, Joo Young; Oh, ChilHwan; You, Seungkwon
October 2008
Journal of Investigative Dermatology;Oct2008, Vol. 128 Issue 10, p2429
Academic Journal
Keloids are benign skin tumors characterized by collagen accumulation and hyperproliferation of fibroblasts. To find an effective therapy for keloids, we explored the pharmacological potential of (−)-epigallocatechin-3-gallate (EGCG), a widely investigated tumor-preventive agent. When applied to normal and keloid fibroblasts (KFs) in vitro, proliferation and migration of KFs were more strongly suppressed by EGCG than normal fibroblast proliferation and migration (IC50: 54.4 μM (keloid fibroblast (KF)) versus 63.0 μM (NF)). The level of Smad2/3, signal transducer and activator of transcription-3 (STAT3), and p38 phosphorylation is more enhanced in KFs, and EGCG inhibited phosphorylation of phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated protein kinase 1/2 (ERK1/2), and STAT3 (Tyr705 and Ser727). To evaluate the contribution of these pathways to keloid pathology, we treated KFs with specific inhibitors for PI3K, ERK1/2, or STAT3. Although a PI3K inhibitor significantly suppressed proliferation, PI3K and MEK/ERK inhibitors had a minor effect on migration and collagen production. However, a JAK2/STAT3 inhibitor and a STAT3 siRNA strongly suppressed proliferation, migration, and collagen production by KFs. We also found that treatment with EGCG suppressed growth and collagen production in the in vivo keloid model. This study demonstrates that EGCG suppresses the pathological characteristics of keloids through inhibition of the STAT3-signaling pathway. We propose that EGCG has potential in the treatment and prevention of keloids.Journal of Investigative Dermatology (2008) 128, 2429–2441; doi:10.1038/jid.2008.103; published online 8 May 2008


Related Articles

  • Chemoprevention Degree of Polyphenon E from Green Tea in Chemically Induced Mammary Tumors in Sprague-Dawley Female Rats.  // Bulletin of the University of Agricultural Sciences & Veterinary;2011, Vol. 68 Issue 1, p389 

    The article presents a research study regarding the effectiveness of Polyphenon E derived from green tea in treating Sprague-Dawley female rats from Cantacuzino Institute in Romania. Study shows the halting of mammary tumor growth in rats administered with 0.5% of Polyphenon E on their drinking...

  • Optimal Management of Skin Cancer in Immunosuppressed Patients. Brin, Lauren; Zubair, Adeel; Brewer, Jerry // American Journal of Clinical Dermatology;Aug2014, Vol. 15 Issue 4, p339 

    Skin cancer is the most common malignancy in humans with basal cell carcinoma representing the majority of cases in the general population. The prevalence of skin cancer is increased amongst immunosuppressed patients such as those with lymphoproliferative disorders including non-Hodgkin lymphoma...

  • ORIGINAL ARTICLE Topically Applied Imiquimod Inhibits Vascular Tumor Growth In Vivo. Sidbury, Robert; Neuschler, Nicole; Neuschler, Erin; Ping Sun; Xiao-qi Wang, Erin; Miller, Richard; Tomai, Mark; Puscasiu, Elena; Gugneja, Sajiv; Paller, Amy S. // Journal of Investigative Dermatology;Nov2003, Vol. 121 Issue 5, p1205 

    Vascular tumors occur in approximately 10% of all infants and may be associated with significant morbidity. Available therapies for vascular tumors, such as systemic corticosteroids, vincristine, and interferon-α, may cause toxicity, limiting their use to complicated cases. Using a mouse...

  • Herpes could combat melanoma.  // Lamp;Jul2015, Vol. 72 Issue 6, p9 

    No abstract available.

  • Green Tea Extract and (−)-Epigallocatechin-3-Gallate Inhibit Mast Cell-Stimulated Type I Collagen Expression in Keloid Fibroblasts via Blocking PI-3K/Akt Signaling Pathways. Qunzhou Zhang; Kelly, A. Paul; Wang, Lina; French, Samuel W.; Xudong Tang; Duong, Hai S.; Messadi, Diana V.; Le, Anh D. // Journal of Investigative Dermatology;Dec2006, Vol. 126 Issue 12, p2607 

    Keloid, a chronic fibro-proliferative disease, exhibits distinctive histological features characterized by an abundant extracellular matrix stroma, a local infiltration of inflammatory cells including mast cells (MCs), and a milieu of enriched cytokines. Previous studies have demonstrated that...

  • EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells. Jung, Y D; Kim, M S; Shin, B A; Chay, K O; Ahn, B W; Liu, W; Bucana, C D; Gallick, G E; Ellis, L M // British Journal of Cancer;3/15/2001, Vol. 84 Issue 6, p844 

    Catechins are key components of teas that have antiproliferative properties. We investigated the effects of green tea catechins on intracellular signalling and VEGF induction in vitro in serum-deprived HT29 human colon cancer cells and in vivo on the growth of HT29 cells in nude mice. In the in...

  • Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation. Meeran, Syed M.; Akhtar, Suhail; Katiyar, Santosh K. // Journal of Investigative Dermatology;May2009, Vol. 129 Issue 5, p1258 

    Consumption of green tea polyphenols (GTPs) in drinking water prevents photocarcinogenesis in mice; however, the molecular mechanisms underlying this effect have not been fully elucidated. Using IL-12p40 knockout (KO) mice and their wild-type counterparts and an established photocarcinogenesis...

  • Brown bumps persist on chest and trunk. Cohen, Bernard A. // Contemporary Pediatrics;Jul2010, Vol. 27 Issue 7, p34 

    The article presents the case of a 15-year-old girl who has brown bumps on her neck, trunk and chest that have spread over the last 8 years. According to the article, the patient has syringoma which is a benign eccrine tumor that occurs in multiples in the periorbital area as well as forehead,...

  • I.S.D.S. Videotapes Now Available.  // Journal of Dermatologic Surgery & Oncology;Feb82, Vol. 8 Issue 2, p109 

    The International Society for Dermatologic Surgery, in cooperation with Schering Corp., is now offering a series of videotapes available for personal loan to members of the American Society for Dermatologic Surgery. Copies of these tapes were recently distributed to all institutions conducting...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics