Stretching DNA with electric fields beneath submicron interfacial constriction created by a closely fitting microdroplet in a microchannel

Hsieh, Shu-Fu; Chang, Chia-Pu; Juang, Yi-Je; Wei, Hsien-Hung
August 2008
Applied Physics Letters;8/25/2008, Vol. 93 Issue 8, p084103
Academic Journal
In this article, we report an alternative strategy for stretching single DNA molecules with electric fields. The approach invokes a closely fitting microdroplet in a microchannel, creating a natural converging geometry for stretching DNA within a submicron interfacial constriction. We demonstrate that DNA molecules can undergo prestretching within the constriction, pseudotethering entropic trap, and rapid extension within the thin film underneath the droplet. An elastic dumbbell model is derived to account for the observed stretch behavior, with predictions in good agreement with the experimental results.


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