The genotypic characterisation of Acanthamoeba isolates from human ocular samples

Yera, H.; Zamfir, O.; Bourcier, T.; Viscogliosi, E.; Noël, C.; Dupouy-Camet, J.; Chaumeil, C.
August 2008
British Journal of Ophthalmology;Aug2008, Vol. 92 Issue 8, p1139
Academic Journal
Background: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis. Methods: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis. Results: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis. Conclusion: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.


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