Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish

Nakamura, Yukino; Suga, Koushirou; Sakakura, Yoshitaka; Sakamoto, Takashi; Hagiwara, Atsushi
September 2008
Canadian Journal of Zoology;Sep2008, Vol. 86 Issue 9, p976
Academic Journal
The populations of the only known self-fertilizing vertebrate Kryptolebias marmoratus (Poey, 1880) (formerly known as Rivulus marmoratus Poey, 1880; Cyprinodontiformes: Aplocheilidae) usually consist of different homozygous lineages; however, heterozygous individuals are found occasionally and ratios of homozygosity and heterozygosity in populations are dependent on the proportion of males. However, it is still unclear what impact male-mediated genetic diversity has on the phenotype of K. marmoratus. To clarify this, we attempted outcrossing between male and hermaphrodite of two different clonal strains with different life-history traits using artificial insemination, and examined the genotypes and growth of the hybrid F2 generation. We detected genetic differences between the two clonal strains using amplified fragment length polymorphism (AFLP) analysis with 3 primer combinations, and then obtained 11 AFLP markers. From a total of 31 artificial inseminations with two clonal strains, 1 of 13 hatched fish clearly indicated heterozygosity. The hybrid F2 generations were also heterozygous. Moreover, the growths of the hybrid F2 generation were intermediate of the parental strains from days 0 to 30. Therefore, outcrossing changes genetic architecture and the new genotypes potentially result in new phenotypes of the subsequent generations of K. marmoratus. It may also play a role in adaptation to new environments and the facilitation of local adaptation. Les populations du seul vertébré connu capable d’autofécondation Kryptolebias marmoratus (Poey, 1880) (désigné antérieurement Rivulus marmoratus Poey, 1880; Cyprinodontiformes : Aplocheilidae) consistent généralement en différentes lignées homozygotes; cependant, on trouve à l’occasion des individus hétérozygotes et les rapports de l’homozygotie et de l’hétérozygotie dans les populations dépendent de la proportion de mâles. Cependant, il n’est pas clair quel impact la diversité génétique causée par les mâles a sur le phénotype de K. marmoratus. Afin d’éclaircir la question, nous avons tenté à l’aide de l’insémination artificielle de faire des croisements exogames entre des mâles et des hermaphrodites de deux différentes souches clonales possédant des traits de cycle biologique différents; nous avons ensuite examiné les génotypes et la croissance de la génération hybride de F2. Nous détectons des différences génétiques entre les deux souches clonales à l’aide d’une analyse du polymorphisme de la longueur des fragments amplifiés (AFLP) avec une combinaison de 3 amorces et nous obtenons 11 marqueurs AFLP. Dans un ensemble de 31 inséminations artificielles avec les deux souches clonales, 1 des 13 poissons éclos montrait des signes évidents d’hétérozygotie. Les générations hybrides de F2 sont aussi hétérozygotes. De plus, la croissance de la génération hybride de F2 est intermédiaire entre celles des souches parentales des jours 0 à 30. Ainsi, les croisements exogames changent l’architecture génétique et les nouveaux génotypes produisent potentiellement de nouveaux phénotypes dans les générations subséquentes de K. marmoratus. Ils peuvent aussi jouer un rôle dans l’adaptation aux nouveaux environnements et l’amélioration de l’adaptation locale.


Related Articles

  • With a little help from science, panda has blessed event  // Smithsonian;Sep1979, Vol. 10 Issue 6, p44 

    No abstract available.

  • Panda made in China.  // Asian Pacific Post;7/30/2009, p10 

    This article deals with the success of China in producing the first giant panda born following artificial conception with frozen sperm.

  • TRACKS.  // Backpacker;Oct2004, Vol. 32 Issue 8, p35 

    Presents an update on issues related to endangered species in the U.S. as of October 2004, including the artificial insemination of a black-footed ferret.

  • Marsupial firsts.  // Australian Geographic;Jan-Mar99, Issue 53, p121 

    Focuses on the first koala which was conceived through artificial insemination by researchers at Lone Pine Koala Sanctuary and the University of Queensland on May 11, 1998. Objectives of the University of Sydney and its allied organizations to use the technology in reproducing marsupial.

  • Notes on the induced reproduction and development of the tigerfish, Hydrocynus vittatus (Characidae), embryos and larvae Deacon, A. R.; du Preez, H. H.; Gagiano, C. L.; Steyn, G. J. // Environmental Biology of Fishes;Dec1996, Vol. 47 Issue 4, p387 

    No abstract available.

  • Prized Pachyderm. Maksel, Rebecca // Smithsonian;Feb2002, Vol. 32 Issue 11, p33 

    Reports on the birth of an elephant calf, Kandula, after its mother, Shanthi, was artificially inseminated at the National Zoo in Washington, D.C. How the calf is only the fourth elephant born in the United States using artificial insemination and the fifth worldwide; Lack of natural breeding...

  • The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture. Kagawa, Hirohiko; Tanaka, Hideki; Ohta, Hiromi; Unuma, Tatsuya; Nomura, Kazuharu // Fish Physiology & Biochemistry;Jun2005, Vol. 31 Issue 2/3, p193 

    The eel has long been esteemed as an important food fish in the world, especially in Japan, and has been used as an experimental fish for many fields of fish physiology. However, the decreases in eel resources have been a serious concern in recent years. The catches of glass eels as seedlings...

  • Some Diagnosis and Therapy Techniques in Rainbow Trout (Oncorhynchus Mykiss) Furunculosis, in Intensive System Bred. Negrea, Octavian; Mireşan, Vioara; Chirilă, Flore; Miclăuş, Viorel; Răducu, Camelia; Bidian, Altina // Scientific Papers: Animal Science & Biotechnologies / Lucrari St;Dec2010, Vol. 43 Issue 2, p44 

    Clinical and paraclinical investigations (morpho-pathologic, bacterioscopic, bacteriologic and sensitiveness test to antibodies) done on 20 fish (rainbow trout), dead or in agony state, taken from a furunculosis pesthole in Salmonidae and proceeded from an intensive exploitation unit of trout,...

  • Preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration Temperature. Parmar, V. R.; Suthar, B. N.; Sharma, V. K.; Nakhashi, H. C.; Parikh, S. S. // Veterinary World;May2012, Vol. 5 Issue 5, p294 

    The study was done to understand the preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration temperature. The washed spermatozoa from 78 semen ejaculates, 26 each from 3 Mehsana bucks, were preserved at 4 ± 10C temperature in SM and TCFEY diluents up to 72 hours and the physical...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics