TITLE

Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish

AUTHOR(S)
Nakamura, Yukino; Suga, Koushirou; Sakakura, Yoshitaka; Sakamoto, Takashi; Hagiwara, Atsushi
PUB. DATE
September 2008
SOURCE
Canadian Journal of Zoology;Sep2008, Vol. 86 Issue 9, p976
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The populations of the only known self-fertilizing vertebrate Kryptolebias marmoratus (Poey, 1880) (formerly known as Rivulus marmoratus Poey, 1880; Cyprinodontiformes: Aplocheilidae) usually consist of different homozygous lineages; however, heterozygous individuals are found occasionally and ratios of homozygosity and heterozygosity in populations are dependent on the proportion of males. However, it is still unclear what impact male-mediated genetic diversity has on the phenotype of K. marmoratus. To clarify this, we attempted outcrossing between male and hermaphrodite of two different clonal strains with different life-history traits using artificial insemination, and examined the genotypes and growth of the hybrid F2 generation. We detected genetic differences between the two clonal strains using amplified fragment length polymorphism (AFLP) analysis with 3 primer combinations, and then obtained 11 AFLP markers. From a total of 31 artificial inseminations with two clonal strains, 1 of 13 hatched fish clearly indicated heterozygosity. The hybrid F2 generations were also heterozygous. Moreover, the growths of the hybrid F2 generation were intermediate of the parental strains from days 0 to 30. Therefore, outcrossing changes genetic architecture and the new genotypes potentially result in new phenotypes of the subsequent generations of K. marmoratus. It may also play a role in adaptation to new environments and the facilitation of local adaptation. Les populations du seul vertébré connu capable d’autofécondation Kryptolebias marmoratus (Poey, 1880) (désigné antérieurement Rivulus marmoratus Poey, 1880; Cyprinodontiformes : Aplocheilidae) consistent généralement en différentes lignées homozygotes; cependant, on trouve à l’occasion des individus hétérozygotes et les rapports de l’homozygotie et de l’hétérozygotie dans les populations dépendent de la proportion de mâles. Cependant, il n’est pas clair quel impact la diversité génétique causée par les mâles a sur le phénotype de K. marmoratus. Afin d’éclaircir la question, nous avons tenté à l’aide de l’insémination artificielle de faire des croisements exogames entre des mâles et des hermaphrodites de deux différentes souches clonales possédant des traits de cycle biologique différents; nous avons ensuite examiné les génotypes et la croissance de la génération hybride de F2. Nous détectons des différences génétiques entre les deux souches clonales à l’aide d’une analyse du polymorphisme de la longueur des fragments amplifiés (AFLP) avec une combinaison de 3 amorces et nous obtenons 11 marqueurs AFLP. Dans un ensemble de 31 inséminations artificielles avec les deux souches clonales, 1 des 13 poissons éclos montrait des signes évidents d’hétérozygotie. Les générations hybrides de F2 sont aussi hétérozygotes. De plus, la croissance de la génération hybride de F2 est intermédiaire entre celles des souches parentales des jours 0 à 30. Ainsi, les croisements exogames changent l’architecture génétique et les nouveaux génotypes produisent potentiellement de nouveaux phénotypes dans les générations subséquentes de K. marmoratus. Ils peuvent aussi jouer un rôle dans l’adaptation aux nouveaux environnements et l’amélioration de l’adaptation locale.
ACCESSION #
34173108

 

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