Role of bone marrow-derived cells in experimental chronic pancreatitis

Marrache, F.; Pendyala, S.; Bhagat, G.; Betz, K. S.; Song, Z.; Wang, T. C.
August 2008
Gut;Aug2008, Vol. 57 Issue 8, p1113
Academic Journal
Background: Chronic pancreatitis is a known risk factor for pancreatic adenocarcinoma. Recent work has pointed to a role for bone marrow-derived progenitor cells (BMDCs) in chronic inflammation-based carcinogenesis. Consequently, the role of BMDCs in chronic pancreatitis was investigated. Methods: The fate of BMDCs was followed using green fluorescent protein and the Y chromosome as bone marrow markers in gender-mismatched transplanted mice treated with repeated injections of cerulein for up to 45 weeks. The phenotype of engrafted BMDCs was assessed based on the co-expression of bone marrow and pancreatic markers. Results: After 45 weeks of cerulein treatment, mice developed severe chronic pancreatitis but no preneoplastic lesions. BMDCs did engraft in the pancreas. Most of the BMDCs were desmin positive and contributed to 5.12% (1.12%) (mean (SEM)) of the pancreatic stellate cell population. Pancreatic stellate cells derived from the bone marrow could be activated, as demonstrated by α-smooth muscle actin expression, suggesting a role in tissue repair. BMDCs could also be found in pancreatic ducts, based on dolichos biflorus agglutinin and cytokeratin 19 stainings, but at a much lower frequency (0.62% (0.11%)). Conclusion: BMDCs contribute to the pancreatic stellate cell population, suggesting a role in pancreatic tissue repair. In the absence of preneoplastic lesions, BMDCs contribute at a very low level to the ductal epithelium of the chronically inflamed pancreas. The role of BMDCs in pancreatic carcinogenesis remains to be defined.


Related Articles

  • Revascularization of ischemic tissues by PDGF-CC via effects on endothelial cells and their progenitors. Xuri Li; Tjwa, Marc; Moons, Lieve; Fons, Pierre; Noel, Agnes; Ny, Annelii; Jian Mm Zhou; Lennartsson, Johan; Hong Li; Luttun, Aernout; Pontén, Annica; Devy, Laetitia; Bouché, Ann; Hideyasu Oh; Manderveld, Ann; Blacher, Silvia; Communi, David; Savi, Pierre; Bono, Françoise; Dewerchin, Mieke // Journal of Clinical Investigation;Jan2005, Vol. 115 Issue 1, p118 

    The angiogenic mechanism and therapeutic potential of PDGF-CC, a recently discovered member of the VEGF/ PDGF superfamily, remain incompletely characterized. Here we report that PDGF-CC mobilized endothelial progenitor cells in ischemic conditions; induced differentiation of bone marrow cells...

  • Host bone-marrow cells are a source of donor intimal smooth- muscle?like cells in murine aortic transplant arteriopathy. Shimizu, Koichi; Sugiyama, Seigo; Aikawa, Masanori; Fukumoto, Yoshihiro; Rabkin, Elena; Libby, Peter; Mitchell, Richard N. // Nature Medicine;Jun2001, Vol. 7 Issue 6, p738 

    Long-term solid-organ allografts typically develop diffuse arterial intimal lesions (graft arterial disease; GAD), consisting of smooth-muscle cells (SMC), extracellular matrix and admixed mononuclear leukocytes. GAD eventually culminates in vascular stenosis and ischemic graft failure. Although...

  • Leiomyoma Cutis of the Cheek. Bart, Robert S.; Kopf, Alfred W. // Journal of Dermatologic Surgery & Oncology;Feb82, Vol. 8 Issue 2, p98 

    Leiomyomata of the skin fall into three main groups, depending on the smooth muscle structure from which they develop. Nontender leiomyomata may develop as isolated masses in the smooth muscles of scrotum. Single nodules, frequently tender may develop in the smooth muscle of vessel walls and are...

  • CASE 1: ARMED WITH A PROBLEM. McGovern, Thomas W. // Cortlandt Forum;7/25/2001, Vol. 14 Issue 7, p25 

    Presents a dermatology case of a patient diagnosed with piloleiomyomas. Benign smooth-muscle tumors arising from the arrectores pilorum muscles of hair follicles; History of painful nodules on left shoulder and lateral arm; Physical examination results; Treatment of solitary lesions.

  • FDA approves thrombopoiesis-stimulating agent. Thompson, Cheryl A. // American Journal of Health-System Pharmacy;10/1/2008, Vol. 65 Issue 19, p1788 

    The article offers information on the approval by the Food and Drug Administration and Amgen Inc., on the use of romiplostim or Nplate. Romiplostim is a thrombospoiesis-stimulating agent, that directly stimulates the bone marrow to produce platelets. The drug is only allowed for used to patients...

  • Development of a new method in promoting fracture healing: multiple cryopreserved bone marrow injections using a rabbit model. Ma, Hsiao-Li; Chen, Tain-Hsiung; Hung, Shih-Chieh // Archives of Orthopaedic & Trauma Surgery;Sep2004, Vol. 124 Issue 7, p448 

    Introduction: It was hypothesized that multiple injections of cryopreserved bone marrow (BM) would promote fracture healing. Materials and methods: Experiments were performed to select a method for freezing BM nucleated cells based on the recovery rate, and to investigate the effects of frozen...

  • Presence of bone-marrow- and neural-crest-derived cells in intimal hyperplasia at the time of clinical in-stent restenosis. Skowasch, Dirk; Jabs, Alexander; Andrié, René; Dinkelbach, Sabine; Lüderitz, Berndt; Bauriedel, Gerhard // Cardiovascular Research;Dec2003, Vol. 60 Issue 3, p684 

    Objective: Intralesional data of coronary target lesions following stent implantation are infrequent. In addition, there is ongoing controversy on the origin of neointimal cells. In this respect, several lines of evidence revealed bone-marrow-derived endothelial progenitor and dendritic cells...

  • Bone marrow lesions: a universal bone response to injury? Eriksen, Erik; Ringe, Johan // Rheumatology International;Mar2012, Vol. 32 Issue 3, p575 

    The ever increasing use of magnetic resonance imaging in clinical practice has led to the recognition of a new entity, bone marrow lesions (BMLs). These lesions are characterized by excessive water signals in the marrow space and have emerged as a central component of many different diseases...

  • Pre-transplant risk factors for cryptogenic organizing pneumonia/bronchiolitis obliterans organizing pneumonia after hematopoietic cell transplantation. Nakasone, H; Onizuka, M; Suzuki, N; Fujii, N; Taniguchi, S; Kakihana, K; Ogawa, H; Miyamura, K; Eto, T; Sakamaki, H; Yabe, H; Morishima, Y; Kato, K; Suzuki, R; Fukuda, T // Bone Marrow Transplantation;Oct2013, Vol. 48 Issue 10, p1368 

    A correction to the article "Bone Marrow Transplantation" that was published in the August 12, 2013 issue is presented.


Read the Article


Sign out of this library

Other Topics