A three-dimensional ultrasonic cage for characterization of individual cells

Manneberg, Otto; Vanherberghen, Bruno; Svennebring, Jessica; Hertz, Hans M.; Önfelt, Björn; Wiklund, Martin
August 2008
Applied Physics Letters;8/11/2008, Vol. 93 Issue 6, p063901
Academic Journal
We demonstrate enrichment, controlled aggregation, and manipulation of microparticles and cells by an ultrasonic cage integrated in a microfluidic chip compatible with high-resolution optical microscopy. The cage is designed as a dual-frequency resonant filleted square box integrated in the fluid channel. Individual particles may be trapped three dimensionally, and the dimensionality of one-dimensional to three-dimensional aggregates can be controlled. We investigate the dependence of the shape and position of a microparticle aggregate on the actuation voltages and aggregate size, and demonstrate optical monitoring of individually trapped live cells with submicrometer resolution.


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