TITLE

Low dose cyclophosphamide rescues myocardial function from ischemia-reperfusion in rats

AUTHOR(S)
Zhu, Jianhua; Qiu, Yuangang; Wang, Qiqi; Zhu, Yujuan; Hu, Shenjiang; Zheng, Liangrong; Wang, Lihong; Zhang, Yun
PUB. DATE
September 2008
SOURCE
European Journal of Cardio-Thoracic Surgery;Sep2008, Vol. 34 Issue 3, p661
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract: Objectives: The effect of low dose of cyclophosphamide (CP) protecting cardiac function from ischemia-reperfusion injury was studied on rats. The premise is that CP inhibits immune and inflammatory process, thereby limits I/R injury and improves myocardial function. Methods: Open chest rats were submitted to 30min of ischemia followed by 3h, 12h or 24h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 time-point subgroups (3h, 12h and 24h; n =8 for each subgroup). A total of 750mg/m2 cyclophosphamide was intraperitoneally administrated in CP group and saline was given to I/R group. A polyethylene tube was inserted into the left ventricular cavity to detect left ventricular systolic pressure (LVSP) and ±dp/dt max. At the end of the experiment, hearts were harvested for histopathological assessment and infarct size determination. Results: Compared with I/R group, rats treated with low dose CP showed a significant recovery in myocardial function with improved LVSP (88±11 vs 69±11mmHg of 3h; 92±11 vs 64±14 mmHg of 12h; 90±11 vs 64±14mmHg of 24h; p <0.01 respectively). The ±dp/dt max also had the similar trends. The myocardial infarct size was reduced in CP group compared to that in I/R group; the infiltration of polymorph nuclear leukocytes (PMNs) in myocardium was decreased in CP group. The histopathological damage score was also attenuated. Conclusions: These findings suggest that low dose CP rescues cardiac function from ischemia-reperfusion injury by alleviating histopathological damage in rat myocardium.
ACCESSION #
33887355

 

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