Vaccination with Bacille-Calmette Guérin Promotes Mycobacterial Control in Guinea Pig Macrophages Infected In Vivo

Ly, Lan H.; Barhoumi, Rola; Cho, Sang H.; Franzblau, Scott G.; McMurray, David N.
September 2008
Journal of Infectious Diseases;9/1/2008, Vol. 198 Issue 5, p768
Academic Journal
Tuberculosis pleurisy was induced by inoculation of virulent (H37Rv strain or Erdman strain) or attenuated (H37Ra strain) green-fluorescent protein-expressing Mycobacterium tuberculosis into guinea pigs that had or had not been vaccinated with Bacille-Calmette Guérin (BCG). Pleural fluid and cells were analyzed for phagosome-lysosome (P-L) fusion, on the basis of confocal microscopy, intracellular and extracellular bacterial survival, and production of cytokine mRNA. BCG vaccination increased fluid volume and cellular accumulation, significantly enhanced P-L fusion, and significantly decreased intracellular bacterial survival in pleural-effusion macrophages of the guinea pigs infected with the 2 virulent strains. Furthermore, significant increases in interferon-γ, transforming growth factor-α, tumor necrosis factor-β, and interleukin-12p40 cytokine mRNA were seen in the pleural cells of the BCG-vaccinated guinea pigs.


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