TITLE

Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip

AUTHOR(S)
Henares, Terence G.; Mizutani, Fumio; Sekizawa, Ryuichi; Hisamoto, Hideaki
PUB. DATE
August 2008
SOURCE
Analytical & Bioanalytical Chemistry;Aug2008, Vol. 391 Issue 7, p2507
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Single-drop analysis of two different real sample solutions (2 µL) while simultaneously monitoring the activity of two sets of ten different proteases on a single microfluidic device is presented. The device, called a capillary-assembled microchip (CAs-CHIP), is fabricated by embedding square glass sensing capillaries (reagent-release capillaries, RRC) in the polydimethylsiloxane (PDMS) lattice microchannel, and used for that purpose. First, the performance reliability was evaluated by measuring the fluorescence response of twenty caspase-3-sensing capillaries on a single CAs-CHIP, and a relative standard deviation of 1.5–8.2 (% RSD, n = 5 or 10) was obtained. This suggests that precise multiplexed protease-activity sensing is possible by using a single CAs-CHIP with multiple RRCs embedded. Then, using a single CAs-CHIP, real sample analysis of the activity of ten different caspases/proteases in cervical cancer (HeLa) cell lysate treated and untreated with the cell-death-inducer drug, doxorubicin, was simultaneously carried out, and a significant difference in enzyme activity between these two samples was observed. These results suggested the usefulness of the CAs-CHIP in the field of drug discovery. [Figure not available: see fulltext.]
ACCESSION #
33372855

 

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