DNA linearization through confinement in nanofluidic channels

Douville, Nicholas; Dongeun Huh; Takayama, Shuichi
August 2008
Analytical & Bioanalytical Chemistry;Aug2008, Vol. 391 Issue 7, p2395
Academic Journal
Stretching DNA has emerged as a vital process for studying the physical and biological properties of these molecules. Over the past decade, there has been increasing research interest in utilizing nanoscale fluidic channels to confine and stretch single DNA molecules. Nanofabricated systems for linearizing DNA have revealed new and important insights into the conformation changes of DNA molecules. They also have emerged as innovative techniques for efficiently separating DNA molecules based on size and for physically mapping genetic information along the genome. This review describes physical theories of DNA linearization, current DNA stretching techniques based on nanofabricated channels, and breakthroughs resulting from the use of nanofluidic channels for DNA linearization.


Related Articles

  • Analysis of T-DNA-Xa21 loci and bacterial blight resistance effects of the transgene Xa21 in transgenic rice. Zhai, Wenxue; Chen, Caiyan; Zhu, Xuefeng; Chen, Xuewei; Zhang, Dechun; Li, Xiaobing; Zhu, Lihuang // Theoretical & Applied Genetics;Aug2004, Vol. 109 Issue 3, p534 

    The genetic loci and phenotypic effects of the transgene Xa21, a bacterial blight (BB) resistance gene cloned from rice, were investigated in transgenic rice produced through an Agrobacterium-mediated transformation system. The flanking sequences of integrated T-DNAs were isolated from Xa21...

  • Secondary uses and the governance of de-identified data: Lessons from the human genome diversity panel.  // BMC Medical Ethics;2011, Vol. 12 Issue 1, p16 

    The article focuses on a study which explored the secondary uses of one de-identified DNA sample collection. English-language scientific articles published between 2002 and 2009 that reported analysis of HGDP Diversity Panel were identified using a combination of keyword and cited reference...

  • Physical delimitation of the pepper Bs3 resistance gene specifying recognition of the AvrBs3 protein from Xanthomonas campestris pv. vesicatoria. Jordan, Tina; Römer, Patrick; Meyer, Annett; Szczesny, Robert; Pierre, Michele; Piffanelli, Pietro; Bendahmane, Abdel; Bonas, Ulla; Lahaye, Thomas // Theoretical & Applied Genetics;Sep2006, Vol. 113 Issue 5, p895 

    The pepper ( Capsicum annuum) Bs3 gene confers resistance to avrBs3-expressing strains of the bacterial spot pathogen Xanthomonas campestris pv. vesicatoria. To physically delimit Bs3, a pepper YAC library was screened with two flanking DNA markers that are separated from Bs3 by 1.0 and 1.2 cM,...

  • PASS-bis: a bisulfite aligner suitable for whole methylome analysis of Illumina and SOLiD reads. Campagna, Davide; Telatin, Andrea; Forcato, Claudio; Vitulo, Nicola; Valle, Giorgio // Bioinformatics;Jan2013, Vol. 29 Issue 2, p268 

    Summary: The sequencing of bisulfite-treated DNA (Bi-Seq) is becoming a gold standard for methylation studies. The mapping of Bi-Seq reads is complex and requires special alignment algorithms. This problem is particularly relevant for SOLiD color space, where the bisulfite conversion C/T changes...

  • The impact of whole-genome sequencing on the reconstruction of human population history. Veeramah, Krishna R.; Hammer, Michael F. // Nature Reviews Genetics;Mar2014, Vol. 15 Issue 3, p149 

    Examining patterns of molecular genetic variation in both modern-day and ancient humans has proved to be a powerful approach to learn about our origins. Rapid advances in DNA sequencing technology have allowed us to characterize increasing amounts of genomic information. Although this clearly...

  • The Human Genome Project.  // History of Science & Technology;2004, p709 

    The article provides information on the Human Genome Project proposed in 1984 by Robert Sinsheimer, then chancellor of the University of California at Santa Cruz. The Human Genome Project has determined the complete sequence of about 3,000,000,000 bases in human DNA. The DNA bases are usually...

  • Efficient Genetic Mapping of Single Nucleotide Polymorphisms Based Upon DNA Mismatch Digestion. Rungis, Dainis; Britta Hamberger; Bérubé, Yanik; Wilkin, Jennifer; Bohlmann, Jörg; Ritland, Kermit // Molecular Breeding;Oct2005, Vol. 16 Issue 3, p261 

    A single-strand specific (sss) nuclease, found in extracts of celery juice, can be used to digest heteroduplex DNA and hence identify heterozygous single nucleotide polymorphism (SNP) sites in PCR products. Here we show this method can be used to map specific genes with relative simplicity and...

  • The Brain, Revealed. Lehrer, Jonah; Clugston, David; L-dopa // Wired;Apr2009, Vol. 17 Issue 4, p88 

    The article reports on a project in which scientists at the Allen Institute for Brain Science in Seattle, Washington are working to create a complete genetic map of the brain by using assembly-line robots and new protocols. As reported, scientists will analyze slices of tissue containing...

  • CNV-seq, a new method to detect copy number variation using high-throughput sequencing. Chao Xie; Tammi, Martti T. // BMC Bioinformatics;2009, Vol. 10, Special section p1 

    Background: DNA copy number variation (CNV) has been recognized as an important source of genetic variation. Array comparative genomic hybridization (aCGH) is commonly used for CNV detection, but the microarray platform has a number of inherent limitations. Results: Here, we describe a method to...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics