TITLE

Incubation type Si-based planar ion channel biosensor

AUTHOR(S)
Urisu, Tsuneo; Asano, Toshifumi; Zhang, Zhenlong; Uno, Hidetaka; Tero, Ryugo; Junkyu, Han; Hiroko, Isoda; Arima, Yusuke; Iwata, Hiroo; Shibasaki, Koji; Tominaga, Makoto
PUB. DATE
August 2008
SOURCE
Analytical & Bioanalytical Chemistry;Aug2008, Vol. 391 Issue 8, p2703
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule. [Figure not available: see fulltext.]
ACCESSION #
33337108

 

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