Immunofunctionalisation of gold transducers for bacterial detection by physisorption

Laczka, Olivier; Baldrich, Eva; Del Campo, F. Javier; Muñoz, Francesc Xavier
August 2008
Analytical & Bioanalytical Chemistry;Aug2008, Vol. 391 Issue 8, p2825
Academic Journal
Functionalisation of the sensing surface is a key factor in immunosensor fabrication as it allows target-selective capture and prevents nonspecific adsorption of undesired components. Gold immunofunctionalisation using self-assembled monolayers (SAM) has been widely exploited to this end for the detection of small targets. However, we recently demonstrated that this strategy fails when detecting whole bacteria cells (Baldrich et al., Anal Bioanal Chem 390:1557–1562, ). We now investigate different physisorption-based alternatives using E. coli as the target organism. Our results demonstrate that physisorption generates the appropriate substrate for the specific detection of bacteria on gold surfaces, providing detection limits down to 105 cells mL−1 in an ELISA-type colorimetric assay. Additionally, surface coverage is highly reproducible when assayed by impedance spectroscopy and the inter- and intra-assay coefficients of variation are below 10–15% in all cases. These surfaces were stable, retained functionality and did not suffer from significant biomolecule desorption after 10 days storage in PBS at 37 °C, hence confirming physisorption as a cheap, simple and efficient strategy for the detection of bacteria.


Related Articles

  • Prevalence and Numbers of Escherichia coli O157 on Bovine Hides at a Beef Slaughter Plant. O'Brien, S. B.; Duffy, G.; Carney, E.; Sheridan, J. J.; McDowell, D. A.; Blair, I. S. // Journal of Food Protection;Apr2005, Vol. 68 Issue 4, p660 

    In this study, we investigated the prevalence and numbers of Escherichia coli O157 on bovine hides. Samples (n = 1,500) were collected over a 17-month period (30 samples per week) by sponge swabbing approximately 122-cm2 areas of the bovine rump of slaughtered cattle at an early stage of carcass...

  • Development of a Surface Plasmon Resonance-Based Immunoassay for Listeria monocytogenes. Leonard, Paul; Hearty, Stephen; Wyatt, Gary; Quinn, John; O'Kennedy, Richard // Journal of Food Protection;Apr2005, Vol. 68 Issue 4, p728 

    A polyclonal antibody was produced against Internalin B (InlB)-enriched extract and used to develop an inhibition assay to detect Listeria monocytogenes cells in solution using surface plasmon resonance. The gene sequence encoding for the InlB protein was cloned into a Qiagen pQE-60 vector,...

  • The gold-sulfur interface at the nanoscale. Häkkinen, Hannu // Nature Chemistry;Jun2012, Vol. 4 Issue 6, p443 

    Thiolate-protected gold surfaces and interfaces, relevant for self-assembled monolayers of organic molecules on gold, for passivated gold nanoclusters and for molecule-gold junctions, are archetypal systems in various fields of current nanoscience research, materials science, inorganic chemistry...

  • Hydrogen adsorption capacities of multi-walled boron nitride nanotubes and nanotube arrays: a grand canonical Monte Carlo study. Ahadi, Zohreh; Shadman, Muhammad; Yeganegi, Saeed; Asgari, Farid // Journal of Molecular Modeling;Jul2012, Vol. 18 Issue 7, p2981 

    Hydrogen adsorption in multi-walled boron nitride nanotubes and their arrays was studied using grand canonical Monte Carlo simulation. The results show that hydrogen storage increases with tube diameter and the distance between the tubes in multi-walled boron nitride nanotube arrays. Also,...

  • Adsorption and switching properties of a N-benzylideneaniline based molecular switch on a Au(111) surface. Óvári, László; Ying Luo; Leyssner, Felix; Haag, Rainer; Wolf, Martin; Tegeder, Petra // Journal of Chemical Physics;7/28/2010, Vol. 133 Issue 4, p044707 

    High resolution electron energy loss spectroscopy has been employed to analyze the adsorption geometry and the photoisomerization ability of the molecular switch carboxy-benzylideneaniline (CBA) adsorbed on Au(111). CBA on Au(111) adopts a planar (trans) configuration in the first monolayer (ML)...

  • Reversible strain by physisorption in nanoporous gold. Detsi, E.; Chen, Z. G.; Vellinga, W. P.; Onck, P. R.; De Hosson, J. T. M. // Applied Physics Letters;8/22/2011, Vol. 99 Issue 8, p083104 

    Reversible strain amplitudes up to 0.02% in response to a 15% change in relative humidity were detected in nanoporous gold. We show that the mechanism involved in dimensional changes during physisorption is associated with changes in the surface stress when molecules are adsorbed from the vapor...

  • Kinetics of two-dimensional phase transition of amphiphilic monolayers at the air/water interface. Fainerman, V.B.; Vollhardt, D.; Melzer, V. // Journal of Chemical Physics;7/1/1997, Vol. 107 Issue 1, p243 

    Develops a theoretical model describing the adsorption kinetics of the two-dimensional first-order phase transition in monolayers. Theory including a kinetic model of the phase transition in Langmuir and Gibbs monolayers; Adsorption from bulk solution; Dissociation kinetics of bulk micelles.

  • Kinetics of multilayer deposition: Models without screening. Krapivsky, P. L. // Journal of Chemical Physics;8/1/1992, Vol. 97 Issue 3, p2134 

    The kinetics of irreversible multilayer deposition on one- and two-dimensional uniform substrates was studied. It was assumed that the distribution of sizes of parking objects, intervals in 1D and disks in 2D, have a small-size and a large-size cutoff, l and L, respectively. The general case...

  • Pathogen–pathogen interactions: a comparative study of Escherichia coli interactions with the clinical and environmental isolates of Acanthamoeba. Suk-Yul Jung; Selwa Alsam; Kwang Kim; Naveed Khan // World Journal of Microbiology & Biotechnology;Oct2008, Vol. 24 Issue 10, p2339 

    Abstract  In this study, we compared the interactions of invasive and non-invasive strains of E. coli with clinical and environmental isolates of Acanthamoeba. The environmental isolate of Acanthamoeba exhibited significantly higher association with E. coli compared with the clinical...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics