The reduction of (ImH)[ trans-RuIIICl4(dmso)(Im)] under physiological conditions: preferential reaction of the reduced complex with human serum albumin

Brindell, Malgorzata; Stawoska, Iwona; Supel, Justyna; Skoczowski, Andrzej; Stochel, Grazyna; van Eldik, Rudi
July 2008
Journal of Biological Inorganic Chemistry;Jul2008, Vol. 13 Issue 6, p909
Academic Journal
A systematic study of the reduction of (ImH)[ trans-RuCl4(dmso)(Im)] (NAMI-A; dmso is dimethyl sulfoxide, Im is imidazole), a promising antimetastasing agent, by l-ascorbic acid under physiological conditions is reported. Under blood plasma conditions (pH 7.4, 0.1�0.15 M NaCl , 37 �C) the rapid reduction of trans-[RuIIICl4(dmso)(Im)]- results in the formation of trans-[RuIICl4(dmso)(Im)]2- within seconds, and is followed by successive dissociation of the chloride ligands, whereas neither dmso nor imidazole ligands are released during the reaction. Under our experimental conditions, the formation of the ascorbate dianion is the rate-determining step, and once it has formed it reacts rapidly with NAMI-A. Moreover, the NAMI-A complex is very unstable at physiological pH (7.4); therefore, the hydrolysis of NAMI-A cannot be excluded as a competing reaction. During hydrolysis, aquated derivatives via stepwise dissociation of chloride and dmso ligands are formed, and most of these species have a higher redox potential and are expected to be even more easily reduced by ascorbic acid. Thus, it is very likely that the reduced form of NAMI-A or the reduction products of its hydrolytic derivatives react with albumin. The reaction of reduced NAMI-A with human serum albumin leads to the formation of stable adducts, with a binding efficiency very similar to that of the parent complex, viz., 3.2 � 0.3 and 4.0 � 0.4 mol of Ru(II) and Ru(III) per mole of albumin, respectively, however with a significantly higher reactivity.


Related Articles

  • Kinetics and mechanism of the reduction of (ImH)[ trans-RuCl4(dmso)(Im)] by ascorbic acid in acidic aqueous solution. Brindell, Malgorzata; Piotrowska, Dorota; Shoukry, Azza A.; Stochel, Grazyna; van Eldik, Rudi // Journal of Biological Inorganic Chemistry;Sep2007, Vol. 12 Issue 6, p809 

    A systematic study of the reduction of (ImH)[ trans-RuCl4(dmso)(Im)] (NAMI-A; dmso is dimethyl sulfoxide, Im is imidazole), a promising antimetastasing agent entering phase II clinical trial, by l-ascorbic acid is reported. The rapid reduction of trans-[RuIIICl4(dmso)(Im)]- results in formation...

  • Melting Calcium Deposits with Transdermal Dimethyl Sulfoxide/Ascorbic Acid Mixture. Cobb, Daniel // Journal of Orthomolecular Medicine;2010, Vol. 25 Issue 4, p214 

    The author discusses the efficacy of the use of dimethyl sulfoxide (DMSO) with a mixture of ascorbic acid, a special kind of Vitamin C, to melt his calcium deposits as well as those of his wife. He points out how he became familiar with DMSO and his discovery of his illness. He offers...

  • MORE ENERGY AND LESS DISEASE WITH VITAMIN C AND MSM. Last, Walter // Nexus;Feb/Mar2014, Vol. 21 Issue 2, p13 

    The article discusses the health benefits of using high doses of vitamin C, methylsulphonylmethane or dimethylsulphone (MSM) and dimethyl sulphoxide (DMSO). It includes supplementing with vitamin C, MSM, DMSO and niacinamide (vitamin B3) to reduce fatigue, to increase metabolic oxygen supply,...

  • Dissolution Behavior of Cellulose in IL + DMSO Solvent: Effect of Alkyl Length in Imidazolium Cation on Cellulose Dissolution. Xu, Airong; Cao, Lili; Wang, Bingjun; Ma, Junying // Advances in Materials Science & Engineering;3/23/2015, Vol. 2015, p1 

    Four cellulose solvents including [C2mim][CH3COO] + DMSO, [C4mim][CH3COO] + DMSO, [C6mim][CH3COO] + DMSO, and [C8mim][CH3COO] + DMSO were prepared by adding dimethyl sulfoxide DMSO in 1-ethyl-3-methylimidazolium acetate [C2mim][CH3COO], 1-butyl-3-methylimidazolium acetate [C4mim][CH3COO],...

  • Study of the physicochemical properties of the BSA: flavonoid nanoparticle. Fang, Ru; Jing, Hao; Chai, Zhi; Zhao, Guanghua; Stoll, Serge; Ren, Fazheng; Liu, Fei; Leng, Xiaojing // European Food Research & Technology;Aug2011, Vol. 233 Issue 2, p275 

    method to design a nanoparticle using bovine serum albumin and flavonoid was introduced in this work. Kaempferol and rutin were used here as models of hydrophobic flavonoids. Bovine serum albumin molecules formed nanoparticle with diameter of X-30 nm, promoted by dimethyl sulfoxide and...

  • Mechanism of Interaction Between Hydrolytic Lignin and Poly(acrylonitrile). Sazanov, Yu.; Krutov, S.; Ipatova, Y.; Kosyakov, D.; Kulikova, Ye. // Eurasian Chemico-Technological Journal;2017, Vol. 19 Issue 1, p23 

    Interaction between polyacrylonitrile (PAN) and hydrolytic lignin (THL) in mixed binary solution dimethyl sulfoxide/water (70:30) allowed us to obtain homogeneous forming solution of the THL/PAN composite. This forming solution was used for preparation of fibers and films which served as...

  • Electronic structural investigations of ruthenium compounds and anticancer prodrugs. Harris, Travis V.; Szilagyi, Robert K.; Holman, Karen L. McFarlane // Journal of Biological Inorganic Chemistry;Jul2009, Vol. 14 Issue 6, p891 

    Several RuIII compounds are propitious anticancer agents although the precise mechanisms of action remain unknown. With this paper we start to establish an experimental library of X-ray absorption spectroscopy (XAS) data for ten Ru compounds wherein the ligands [Cl-, dimethyl sulfoxide,...

  • Dimethyl Sulfoxide Promotes the Multiple Functions of the Tumor Suppressor HLJ1 through Activator Protein-1 Activation in NSCLC Cells. Chi-Chung Wang; Sheng-Yi Lin; Yi-Hua Lai; Ya-Jung Liu; Yuan-Lin Hsu; Chen, Jeremy J. W. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Background: Dimethyl sulfoxide (DMSO) is an amphipathic molecule that displays a diversity of antitumor activities. Previous studies have demonstrated that DMSO can modulate AP-1 activity and lead to cell cycle arrest at the G1 phase. HLJ1 is a newly identified tumor and invasion suppressor that...

  • Chromosome aberrations induced by eumaitenine, a sesquiterpene isolated from Maytenus boaria Mol. in cultured CHO cells. García, M.; Duk, S.; Weigert, G.; Becerra, J.; Silva, M.; Alarcón, M. // Bulletin of Environmental Contamination & Toxicology;Dec1993, Vol. 51 Issue 6, p803 

    The article presents a study on the genotoxic characteristics of sesquiterpene Eumaitenine (EUM) from the seeds of Maitenus boaria. It states that the study used cultured Chinese hamster ovary cells (CHO line), dimethyl sulfoxide (DMSO) and ethyl methanesulfonate (EMS). It concludes that these...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics