Novel bioreactors for the culture and expansion of aggregative neural stem cells

Yuen Ng; Howard Chase
August 2008
Bioprocess & Biosystems Engineering;Aug2008, Vol. 31 Issue 5, p393
Academic Journal
Abstract  Neural stem cells have been cultured as three-dimensional aggregates in a number of different types of bioreactors. The design and configuration of the bioreactor are shown to be crucial factors for the successful propagation of the cells. A novel bioreactor with liquid re-circulation and a working volume of 200 ml has been designed, tested and shown to be able to produce a higher cell vitality compared to those produced in multi-well plates, shake flasks and stirred flasks. The novel reactor was able to produce a total density of cells of 3.5 × 106 cells/ml consisting of a larger number of smaller and proliferative aggregates, compared to only 1.8 × 106 cells/ml produced in a multi-well plate. Shake flasks and stirred flasks commonly used for facilitating mass transfer in the culture of micro-organisms are shown to be unsuitable for the propagation of neural stem cells.


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