TITLE

Fibronectin Facilitates the Invasion of Orientia tsutsugamushi into Host Cells through Interaction with a 56-kDa Type-Specific Antigen

AUTHOR(S)
Jung-Hee Lee; Nam-Hyuk Cho; Se-Yoon Kim; Sun-Young Bang; Hyuk Chu; Myung-Sik Choi; and Ik-Sang Kim
PUB. DATE
July 2008
SOURCE
Journal of Infectious Diseases;7/15/2008, Vol. 198 Issue 2, p250
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background. Orientia tsutsugamushi, the causative agent of scrub typhus, is an obligate intracellular bacterium. The pathogen's mechanism of cellular invasion is poorly characterized. Methods. Through ligand immunoblots, glutathione S-transferase (GST) pull-down assays, and in vitro inhibition assays of intracellular invasion, a bacterial ligand was identified and was shown to interact with fibronectin (Fn) to enhance the intracellular invasion of O. tsutsugamushi. Results. O. tsutsugamushi can bind to immobilized Fn in vitro, and exogenous Fn stimulates bacterial invasion of mammalian host cells. Bacterial invasion in the presence of Fn was abrogated by the addition of Arg-Gly-Asp peptides or by an anti-α5β1 integrin antibody. Through a ligand immunoblot and GST pull-down assay, a 56-kDa type-specific antigen (TSA56) was identified as the bacterial ligand responsible for the interaction with Fn. Antigenic domain III and the adjacent C-terminal region (aa 243-349) of TSA56 interacted with Fn. Furthermore, we found that the enhanced invasion of the pathogen was abrogated by the addition of purified recombinant peptides derived from TSA56. Conclusion. Fn facilitates the invasion of O. tsutsugamushi through its interaction with TSA56.
ACCESSION #
33114338

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics