Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles

Xiaojing Zhang; Xing, James Z.; Jie Chen; Ko, Lawrence; Amanie, John; Gulavita, Sunil; Pervez, Nadeem; Yee, Don; Moore, Ronald; Roa, Wilson
June 2008
Clinical & Investigative Medicine;Jun2008, Vol. 31 Issue 3, pE160
Academic Journal
Purpose: Nanotechnology is an emerging field with significant translational potential in medicine. In this study, we applied gold nanoparticles (GNP) to enhance radiation sensitivity and growth inhibition in radiation-resistant human prostate cancer cells. Methods: Gold nanoparticles (GNPs) were synthesized using HAuCl4 as the gold particle source and NaBH4 as the reductant. Either thio-glucose or sodium citrate was then added to the solution separately to bind the GNPs to form thio-glucose-capped gold nanoparticles (Glu-GNP) and neutral gold nanoparticles (TGS-GNPs). Human prostate carcinoma DU-145 cells were exposed to vehicle, irradiation, 15nM TGS-GNPs, or 15nM Glu-GNPs, or GNPs plus irradiation. The uptake assays of GNP were performed using hemocytometer to count cells and the mass spectrometry was applied to calculate gold mass. The cytotoxicity induced by GNPs, irradiation, or GNPs plus irradiation was measured using a standard colorimetric MTT assay. Results: Exposure to Glu-GNPs resulted in a three times increase of nanoparticle uptake compared to that of TGSGNPs in each target cell (p<0.005). Cytoplasmic intracellular uptake of both TGS-GNPs and Glu-GNPs resulted in a growth inhibition by 30.57% and 45.97% respectively, comparing to 15.88% induced by irradiation alone, in prostate cancer cells after exposure to the irradiation. Glu-GNPs showed a greater enhancement, 1.5 to 2 fold increases within 72 hours, on irradiation cytotoxicity compared to TGS-GNPs. Tumour killing, however, did not appear to correlate linearly with nanoparticle uptake concentrations. Conclusion: These results showed that functional glucose-bound gold nanoparticles enhanced radiation sensitivity and toxicity in prostate cancer cells. In vivo studies will be followed to verify our research findings.


Related Articles

  • Focal cryo in prostate Ca spares continence, potency.  // Urology Times;10/15/2006, Vol. 34 Issue 13, p3 

    The article presents the result of the study which reveals the effectiveness of cryoablation in killing cancerous prostate tissue while maintaining sexual and urinary functions. The study employed 31 subjects who were diagnosed with organ-confined unilateral tumor and were exposed to the...

  • Hot Pepper For Prostate Cancer May Hold Promise.  // RN;Jun2006, Vol. 69 Issue 6, Special section p4 

    The article discusses a study on the role of capsaicin in inhibiting the growth of prostate cancer cells. Capsaicin, a component of red peppers, was effective in killing approximately 80% of the cancer cells. The investigators believe capsaicin kills cancer cells through its effects on a key...

  • Technique may aid in evaluating prostate cancer cells. Mulcahy, Nicholas // Urology Times;11/1/2004, Vol. 32 Issue 16, p9 

    Discusses a study about the effectiveness of tumor cell enrichment technique in increasing the detection rate of circulating prostatic tumor cells. Evaluation of cancer in bone marrow samples; Ability of the technique to identify micrometastasis of prostate cancer; Molecular profiling of the...

  • Charcot-Leyden crystals in a prostatic adenocarcinoma. Aydın, Özgür // Diagnostic Pathology;2006, Vol. 1, p26 

    A transrectal needle biopsy from a 63-year-old man was decided because of a high prostatic spesific antigen in the blood, and a hard right lobe in rectal examination. 10 examples were taken from each lobe. In 1 of 4 of the examples from the left lobe, which contained a small focus of...

  • ACIs offer new hope for prostate cancer patients. Blank, Dennis // Drug Topics;6/18/2007, Vol. 151 Issue 12, p16 

    The article reports on the potential of active cellular immunotherapy (ACI) as treatment of prostate cancer in the U.S. It states that ACI utilizes a patient's cells that have been treated to trigger a specific and long-lasting immune response that enables the body to fight cancer cells....

  • Reduced expression and tumor suppressor function of the ETS transcription factor ESE-3 in prostate cancer. Cangemi, R.; Mensah, A.; Albertini, V.; Jain, A.; Mello-Grand, M.; Chiorino, G.; Catapano, C. V.; Carbone, G. M. // Oncogene;5/1/2008, Vol. 27 Issue 20, p2877 

    Deregulated expression of ETS transcription factors has emerged as an important event in prostate cancer pathogenesis. Here we show that the expression of epithelial-specific ETS (ESE)-3 factor is frequently reduced at the RNA and protein level in prostate cancer clinical samples compared to...

  • Identifying New Candidate Genes and Chemicals Related to Prostate Cancer Using a Hybrid Network and Shortest Path Approach. Yuan, Fei; Zhou, You; Wang, Meng; Yang, Jing; Wu, Kai; Lu, Changhong; Kong, Xiangyin; Cai, Yu-Dong // Computational & Mathematical Methods in Medicine;10/4/2015, Vol. 2015, p1 

    Prostate cancer is a type of cancer that occurs in the male prostate, a gland in the male reproductive system. Because prostate cancer cells may spread to other parts of the body and can influence human reproduction, understanding the mechanisms underlying this disease is critical for designing...

  • Adenovirus-mediated IKKβKA expression sensitizes prostate carcinoma cells to TRAIL-induced apoptosis. Sanlioglu, A. D.; Koksal, I. T.; Karacay, B.; Baykara, M.; Luleci, G.; Sanlioglu, S. // Cancer Gene Therapy;Jan2006, Vol. 13 Issue 1, p21 

    Despite the fact that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in cancer cells, TRAIL resistance in cancer cells has challenged the use of TRAIL as a therapeutic agent. First, prostate carcinoma cell lines (DU145, LNCaP and PC3) were...

  • New total PSA and free PSA criteria may reduce unnecessary prostate biopsies.  // Geriatrics;Jun2000, Vol. 55 Issue 6, p22 

    Discusses the results of the study conducted by researchers from Chicago, Illinois on the benefits of prostate cancer detection strategies. Benefits of using free PSA measurements; How the study was conducted; Details on the results of the study.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics