Effects of adrenomedullin on the cell numbers and apoptosis of endothelial progenitor cells

Xiang-Quan Kong; Le-Xin Wang; Chuan-Sheng Yang; Shuang-Feng Chen; Yu-Zeng Xue; Yi-Hua Liu
June 2008
Clinical & Investigative Medicine;Jun2008, Vol. 31 Issue 3, pE117
Academic Journal
Purpose: To investigate the effect of adrenomedullin on the cell numbers and apoptosis of endothelial progenitor cells (EPCs). Methods: Mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation. The cells were stimulated with adrenomedullin, before and after the treatment of adrenomedullin-receptor antagonist, adrenomedullin 22-52, or a PI3K inhibitor LY294002. Results: Adrenomedullin dose-dependently increased the number of EPCs (P<0.05). Adrenomedullin also significantly decreased apoptosis rate of EPCs in a concentration-dependent manner (P<0.05). In the isolated human mononuclear cells pretreated with adrenomedullin 22-52 or LY294002, adrenomedullin failed to increase the number of EPCs or to reduce the level of apoptosis. Conclusions: Adrenomedullin increases the number of EPCs and decreases their apoptosis. These actions are likely mediated by PI3K signaling pathways. The clinical importance of these favourable effects on EPCs remains to be determined.


Related Articles

  • Amylin and Adrenomedullin: Novel Regulators of Bone Growth. Cornish, J.; Naot, D. // Current Pharmaceutical Design;Oct2002, Vol. 8 Issue 23, p2009 

    Bone growth is regulated by circulating hormones and locally generated factors. Understanding their mechanisms of action enables us to obtain a better appreciation of the cellular and molecular basis of bone remodelling, and could therefore be valuable in approaches to new therapies. In this...

  • Adrenomedullin.  // Encyclopedic Reference of Molecular Pharmacology;2004, p16 

    The article presents an encyclopedia entry for adrenomedullin, which refers to a 52-amino acid peptide, belonging to the calcitonin/calcitonin gene-related peptide/amylin peptide family, that it is synthesized mainly in endothelial cells and elicits vasodilation.

  • adrenomedullin.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p53 

    An encyclopedia entry for "adrenomedullin," which refers to a 52-amino acid regulatory peptide that influences a wide variety of body functions, such as blood vessel dilation, cellular growth, circulation, electrolyte balance, kidney function, and neurotransmission, is presented.

  • Receptors.  // Current Medical Literature: GH & Growth Factors;2004, Vol. 19 Issue 3, p80 

    Describes the effects of amylin and adrenomedullin on bone. Mchanism of actions of amylin and adrenomedullin; Significance of insulin-like growth factor (IGF) receptor for mediating IGF production; Influence of IGF-I and amylin/adrenomedullin on osteoblasts.

  • Exogenous adrenomedullin prevents and reverses hypodynamic circulation and pulmonary hypertension in ovine endotoxaemia. C. Ertmer; A. Morelli; S. Rehberg; M. Lange; C. Hucklenbruch; H. Van Aken; M. Booke; M. Westphal // BJA: The British Journal of Anaesthesia;Dec2007, Vol. 99 Issue 6, p830 

    Background Hypodynamic septic shock is associated with a poor prognosis. The present randomized-controlled laboratory experiment was designed to test the hypothesis that the vasodilatory peptide hormone adrenomedullin (ADM) is a useful agent to prevent and reverse the development of hypodynamic...

  • Association study of calcitonin-receptor-like receptor gene in essential hypertension. Sano, Morihiko; Kuroi, Nobuhiro; Nakayama, Tomohiro; Sato, Naoyuki; Izumi, Yoichi; Soma, Masayoshi; Kokubun, Shinichiro // American Journal of Hypertension;Mar2005, Vol. 18 Issue 3, p403 

    Background: Plasma adrenomedullin (ADM) concentrations increase in patients with hypertension, renal failure, heart failure, essential pulmonary hypertension, myocardial infarction, endotoxin shock, and many other conditions. The ADM receptor is a complex molecule that consists of...

  • Adrenomedullin: molecular mechanisms and its role in cardiac disease. B. Yanagawa; N. Nagaya // Amino Acids;Jan2007, Vol. 32 Issue 1, p157 

  • Vinblastine inhibits the angiogenic response induced by adrenomedullin in vitro and in vivo. Ribatti, Domenico; Guidolin, Diego; Conconi, Maria Teresa; Nico, Beatrice; Baiguera, Silvia; Parnigotto, Pier Paolo; Vacca, Angelo; Nussdorfer, Gastone Giovanni // Oncogene;9/25/2003, Vol. 22 Issue 41, p6458 

    Adrenomedullin (ADM) is protumorigenic by stimulating tumor cell growth and angiogenesis. In this context, ADM is identified as a novel target for antiangiogenic therapy. In this study, we addressed the possibility that vinblastine (VBL), as demonstrated in other experimental conditions, may act...

  • The Association of Mid-Regional Pro-Adrenomedullin and Mid-Regional Pro-Atrial Natriuretic Peptide with Mortality in an Incident Dialysis Cohort. Gouya, Ghazaleh; Sturm, Gisela; Lamina, Claudia; Zitt, Emanuel; Freist├Ątter, Otto; Struck, Joachim; Wolzt, Michael; Knoll, Florian; Lins, Friederike; Lhotta, Karl; Neyer, Ulrich; Kronenberg, Florian // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    High levels of the plasma peptides mid-regional pro-adrenomedullin (MR-proADM) and mid-regional pro-atrial natriuretic peptide (MR-proANP) are associated with clinical outcomes in the general population. Data in patients with chronic kidney disease are sparse. We therefore investigated the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics