Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma

June 2008
Croatian Medical Journal;2008, Vol. 49 Issue 3, p453
Academic Journal
No abstract available.


Related Articles

  • Multiplex PCR for 17 Y-Chromosome Specific Short Tandem Repeats (STR) to Enhance the Reliability of Fetal Sex Determination in Maternal Plasma. Yuan Rong; Jiajia Gao; Xinqiang Jiang; Fang Zheng // International Journal of Molecular Sciences;May2012, Vol. 13 Issue 5, p5972 

    The aim of the study was to demonstrate the influence of target gene and amplification product length on the performance of fetal gender determination systems using maternal plasma. A total of 40 pairs of plasma DNA samples from pregnant women and genomic DNA samples from maternal blood,...

  • Detection of male and female fetal DNA in maternal plasma by multiplex fluorescent polymerase chain reaction amplification of short tandem repeats. Pertl, Barbara; Sekizawa, Akihiko; Samura, Osamu; Orescovic, Irmgard; Rahaim, Peter T.; Bianchi, Diana W. // Human Genetics;Jan2000, Vol. 106 Issue 1, p45 

    The purpose of this study was to develop a fluorescent polymerase chain reaction (PCR) assay for the detection of circulating fetal DNA in maternal plasma. Maternal DNA extracted from plasma samples of pregnant women at term and newborn DNA isolated from cord blood were used to genotype 12...

  • Fetal DNA detection in maternal plasma throughout gestation. Galbiati, Silvia; Smid, Maddalena; Gambini, Dania; Ferrari, Augusto; Restagno, Gabriella; Viora, Elsa; Campogrande, Mario; Bastonero, Simona; Pagliano, Marco; Calza, Stefano; Ferrari, Maurizio; Cremonesi, Laura // Human Genetics;Jul2005, Vol. 117 Issue 2/3, p243 

    The presence of fetal DNA in maternal plasma may represent a source of genetic material which can be obtained noninvasively. We wanted to assess whether fetal DNA is detectable in all pregnant women, to define the range and distribution of fetal DNA concentration at different gestational ages,...

  • Non-invasive prenatal detection of achondroplasia using circulating fetal DNA in maternal plasma. Lim, Ji; Kim, Mee; Kim, Shin; Kim, Hye; Song, Mee; Kim, Min; Park, So; Yang, Jae; Ryu, Hyun // Journal of Assisted Reproduction & Genetics;Feb2011, Vol. 28 Issue 2, p167 

    Purpose: To perform a reliable non-invasive detection of the fetal achondroplasia using maternal plasma. Methods: We developed a quantitative fluorescent-polymerase chain reaction (QF-PCR) method suitable for detection of the FGFR3 mutation (G1138A) causing achondroplasia. This method was...

  • Noninvasive Prenatal Diagnosis of Fetal Trisomy 21 by Allelic Ratio Analysis Using Targeted Massively Parallel Sequencing of Maternal Plasma DNA. Liao, Gary J. W.; Chan, K. C. Allen; Jiang, Peiyong; Hao Sun; Leung, Tak Y.; Chiu, Rossa W. K.; Lo, Y. M. Dennis // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Background: Plasma DNA obtained from a pregnant woman contains a mixture of maternal and fetal DNA. The fetal DNA proportion in maternal plasma is relatively consistent as determined using polymorphic genetic markers across different chromosomes in euploid pregnancies. For aneuploid pregnancies,...

  • Prenatal Diagnosis of Fetal RhD Status by Molecular Analysis of Maternal Plasma. Lo, Y.M. Dennis; Hjelm, N. Magnus; Fidler, Carrie; Sargent, Ian L.; Murphy, Michael F.; Chamberlain, Paul F.; Poon, Priscilla M.K.; Redman, Christopher W.G.; Wainscoat, James S. // New England Journal of Medicine;12/10/98, Vol. 339 Issue 24, p1734 

    Background: The ability to determine fetal RhD status noninvasively is useful in the treatment of RhD-sensitized pregnant women whose partners are heterozygous for the RhD gene. The recent demonstration of fetal DNA in maternal plasma raises the possibility that fetal RhD genotyping may be...

  • A Low-Cost Efficient Multiplex P CR for Prenatal Sex Determination in Bovine Fetus Using Free Fetal DNA in Maternal Plasma. Davoudi, Arash; Seighalani, Ramin; Aleyasin, Seyed Ahmad; Tarang, Alireza; Salehi, Abdolreza; Tahmoressi, Farideh // International Journal of Fertility & Sterility;Apr-Jun2012, Vol. 6 Issue 1, p45 

    Background: In order to establish a reliable non-invasive method for sex determination in a bovine fetus in a routine setting, the possibility of identifying specific sequence in the fetal X and Y-chromosomes has been evaluated in maternal plasma using conventional multiplex polymerase chain...

  • A Method to Quantify Cell-Free Fetal DNA Fraction in Maternal Plasma Using Next Generation Sequencing: Its Application in Non-Invasive Prenatal Chromosomal Aneuploidy Detection. Xu, Xu-Ping; Gan, Hai-Yan; Li, Fen-Xia; Tian, Qi; Zhang, Jun; Liang, Rong-Liang; Li, Ming; Yang, Xue-Xi; Wu, Ying-Song // PLoS ONE;1/14/2016, Vol. 11 Issue 1, p1 

    Objective: The fraction of circulating cell-free fetal (cff) DNA in maternal plasma is a critical parameter for aneuploidy screening with non-invasive prenatal testing, especially for those samples located in equivocal zones. We developed an approach to quantify cff DNA fractions directly with...

  • Non-invasive prenatal testing for Down syndrome. Hyett, Jon // Australian Prescriber;Apr2014, Vol. 37 Issue 2, p51 

    Fetal DNA can be detected in maternal plasma. This can be used to identify chromosomal and genetic abnormalities. The concentration of free fetal DNA increases with advancing gestation. Non-invasive prenatal testing should not be performed before 10 weeks. Non-invasive prenatal testing has more...

  • Fetal gender determination in early pregnancy through qualitative and quantitative analysis of fetal DNA in maternal serum. Honda, Hiroshi; Miharu, Norio; Ohashi, Yoko; Samura, Osamu; Kinutani, Masayuki; Hara, Tetsuaki; Ohama, Koso // Human Genetics;Jan2002, Vol. 110 Issue 1, p75 

    Fetal DNA in maternal plasma and serum has been shown to be a useful material for fetal gender determination and for screening tests for abnormal pregnancies except during early gestational ages. Maternal serum samples were obtained from 81 pregnant women during the 5th–10th weeks of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics