TITLE

In vitro assays to test the interference of anti-thyroglobulin antibodies on thyroglobulin measurement

AUTHOR(S)
Deolinda Madureira; Susana Prazeres; Márcia Pedro; Teresa Pereira; Ana Font; Maria Bugalho
PUB. DATE
April 2008
SOURCE
Endocrine (1355008X);Apr2008, Vol. 33 Issue 1, p40
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract   Objective To assess the interference of anti-thyroglobulin antibodies (TgAb) on serum thyroglobulin (Tg) measurement by in vitro experiments. Design Re-evaluation of Tg concentration after dilution with different TgAb-positive sera. On a first step, dilutions of the same Tg with different TgAb sera were performed and on a second step, different Tgs were diluted with the same TgAb serum. Methods Tg measurements were performed using an immunometric (IMA) chemiluminescence assay. TgAb measurements were performed using two methods: immunoflurimetric assay (UNICAP 100) and IMA chemiluminescent assay (IMMULITE 2000). Results Dilution of a known concentration of Tg with different TgAb-positive sera resulted in a variation of the final concentration of Tg ranging from −24 to −79%. A weak correlation was observed between the TgAb concentration and the percentage of the Tg deviation. Dilution of different Tgs with the same TgAb-positive serum illustrated how the same TgAb positive serum may determine a high interference or a neutral effect. Conclusions Present results suggest that the interference on Tg measurement observed in the presence of TgAb may result not only from the anti-thyroglobulin antibodies, but also from the thyroglobulin itself.
ACCESSION #
32951287

 

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