TITLE

Increased capsaicin receptor TRPV1-expressing sensory fibres in irritable bowel syndrome and their correlation with abdominal pain

AUTHOR(S)
Akbar, A.; Yiangou, Y.; Facer, P.; Walters, J. A. F.; Anand, P.; Ghosh, S.
PUB. DATE
July 2008
SOURCE
Gut;Jul2008, Vol. 57 Issue 7, p923
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: The capsaicin receptor TRPV1 (transient receptor potential vanilloid type-1) may play an important role in visceral pain and hypersensitivity states. In irritable bowel syndrome (IBS), abdominal pain is a common and distressing symptom where the pathophysiology is still not clearly defined. TRPV1-immunoreactive nerve fibres were investigated in colonic biopsies from patients with IBS, and this was related to abdominal pain. Methods: Rectosigmoid biopsies were collected from 23 lBS patients fulfilling Rome II criteria, and from 22 controls. Abdominal pain scores were recorded using a validated questionnaire. TRPV1-, substance P- and neuronal marker protein gene product (PGP) 9.5-expressing nerve fibres, mast cells (c-kit) and lymphocytes (CD3 and CD4) were quantified, following immunohistochemistry with specific antibodies. The biopsy findings were related to the abdominal pain scores. Results: A significant 3.5-fold increase in median numbers of TRPV1-immunoreactive fibres was found in biopsies from lBS patients compared with controls (p<0.0001 (. Substance P-immunoreactive fibres (p = 0.01), total nerve fibres (PGP9.5) (p = 0.002), mast cells (c-kit) (p = 0.02) and lymphocytes (CD3( (p = 0.03) were also significantly increased in the lBS group. In multivariate regression analysis, only TRPV1-immunoreactive fibres (p = 0.005) and mast cells (p = 0.008) were significantly related to the abdominal pain score. Conclusions: Increased TRPV1 nerve fibres are observed in IBS, together with a low-grade inflammatory response. The increased TRPV1 nerve fibres may contribute to visceral hypersensitivity and pain in IBS, and provide a novel therapeutic target.
ACCESSION #
32894057

 

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