NIES certified reference material for microcystins, hepatotoxic cyclic peptide toxins from cyanobacterial blooms in eutrophic water bodies

Sano, Tomoharu; Takagi, Hiroo; Nishikawa, Masataka; Kaya, Kunimitsu
July 2008
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 6, p2005
Academic Journal
A certified reference material (CRM) for microcystins has been prepared by the National Institute for Environmental Studies (NIES). Microcystins are hepatotoxic cyclic peptides produced by cyanobacteria in eutrophic water bodies. At least seven microcystin variants were found by HPLC analysis of the NIES CRM, of which [Dha7]microcystin-RR and -LR were the major microcystins present. Because of the lack of available standards we determined the total microcystin concentration in the CRM by the MMPB method, and elucidated the structures of the main individual microcystin variants following their isolation. Analyses of NMR and MS spectra indicated that the remaining minor variants in the CRM were [ D-Asp3, Dha7]microcystin-RR and -LR, and [Dha7]microcystin-YR, -ThTyrR, and -HilR. The CRM is valuable not only as a standard material for the quantitation of total microcystins but also for the identification of individual [Dha7]microcystin variants.


Related Articles

  • LC-MS analyses of microcystins in fish tissues overestimate toxin levels–critical comparison with LC-MS/MS. Kohoutek, J.; Adamovský, O.; Oravec, M.; Šimek, Z.; Palíková, M.; Kopp, R.; Bláha, L. // Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 3, p1231 

    Microcystins are cyclic peptide toxins with hepatotoxic and tumour-promoting properties which are produced in high quantities in freshwater cyanobacterial water blooms, and several studies have reported microcystin accumulation in fish with possible food transfer to humans. In this study, we...

  • Microcystin occurrence in lakes in �land, SW Finland. Lindholm, Tore; Vesterkvist, Pia; Spoof, Lisa; Lundberg-Niinist�, Catharina; Meriluoto, Jussi // Hydrobiologia;Sep2003, Vol. 505 Issue 1-3, p129 

    Samples from a total of 134 lakes, practically all freshwater lakes in �land, SW Finland, were screened for microcystins, hepatotoxic peptides of cyanobacteria, using high performance liquid chromatography (HPLC), time-resolved fluoroimmunoassay (TR-FIA), and protein phosphatase inhibition...

  • Isolation and Characterization of Microcystins (Heptapeptides Hepatotoxins) from Microcystis aeruginosa Bloom in a Homestead Pond, Dhaka, Bangladesh. Ahmed, Sagir // Research Journal of Environmental Sciences;2009, Vol. 3 Issue 2, p245 

    A bloom of Microcystis aeruginosa occurred in a homestead pond in Rupgoanj, Dhaka. Bloom sample was collected and filtered through a glass fiber filter. Methanol-water extract of filtered cells were analyzed by High Performance Liquid Chromatography (HPLC) with UV, MS and MS-MS detection,...

  • Distribution of Microcystins in a Lake Foodweb: No Evidence for Biomagnification. Ibelings, B. W.; Bruning, K.; De Jonge, J.; Wolfstein, K.; Pires, L. M. Dionisio; Postma, J.; Burger, T. // Microbial Ecology;May2005, Vol. 49 Issue 4, p487 

    Microcystins, toxins produced by cyanobacteria, may play a role in fish kills, although their specific contribution remains unclear. A better understanding of the eco-toxicological effects of microcystins is hampered by a lack of analyses at different trophic levels in lake foodwebs. We present...

  • The Presence of Microcystins in Aquatic Ecosystems in Northern Nigeria: Zaria as a Case Study. Chia, A. M.; Abolude, D. S; Ladan, Z.; Akanbi, O.; Kalaboms, A. // Research Journal of Environmental Toxicology;2009, Vol. 3 Issue 4, p170 

    Evidence from research in most countries of the world has shown that Cyanobacteria blooms could exhibit acute and chronic toxicity to man and animals alike. Despite the availability of records from other countries of the world, there is no information on the occurrence of these toxins in...

  • Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Hui Pan; Lirong Song; Yongding Liu; Börner, Thomas // Archives of Microbiology;Dec2002, Vol. 178 Issue 6, p421 

    Microcystins are small hepatotoxic peptides produced by a number of cyanobacteria. They are synthesized non-ribosomally by multifunctional enzyme complex synthetases encoded by the mcy genes. Primers deduced from mcy genes were designed to discriminate between toxic microcystin-producing strains...

  • Genetic Analysis of the Peptide Synthetase Genes for a Cyclic Heptapeptide Microcystin in Microcystis spp.1. Nishizawa, Tomoyasu; Asayama, Munehiko; Fujii, Kiyonaga; Harada, Ken-ichi; Shirai, Makoto // Journal of Biochemistry;1999, Vol. 126 Issue 3, p520 

    Peptide-synthetase-encoding DNA fragments were isolated by a PCR-based approach from the chromosome of Microcystis aeruginosa K-139, which produces cyclic heptapeptides, 7-desmethylmicrocystin-LR and 3, 7-didesmethylmicrocystin-LR. Three open reading frames (mcyA, mcyB, mcyC) encoding...

  • Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis. Zhang, Wei; Liang, Gaodao; Wu, Laiyan; Tuo, Xun; Wang, Wenjing; Chen, Jun; Xie, Ping // Ecotoxicology;Aug2013, Vol. 22 Issue 6, p1012 

    To elucidate the interspecies variation of susceptibility to microcystins (MCs), fresh plasma and purified albumin from six kinds of mammals and fish were used in toxins-substances binding test. Protein contents in the test plasma were analyzed and the binding characteristics to MCs were...

  • Variations in the Microcystin Content of Different Fish Species Collected from a Eutrophic Lake. Schmidt, Justine R.; Shaskus, Mylynda; Estenik, John F.; Oesch, Carl; Khidekel, Roman; Boyer, Gregory L. // Toxins;May2013, Vol. 5 Issue 5, p992 

    Microcystins produced from cyanobacteria can accumulate in fish tissues. Liquid chromatography coupled with tandem quadrupole mass spectrometry (LC-MS/MS) is an attractive alternative to immunoassays for the determination of low concentrations of microcystins in tissues. Fish taken from Grand...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics