Detecting mitochondrial RNA and other cellular events in living cells

Umezawa, Yoshio
July 2008
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1591
Academic Journal
Intracellular signaling can be monitored in vivo in living cells by genetically encoded intracellular fluorescent probes. In this review, three aspects of these probes are introduced: 1) the imaging dynamics of endogenous mitochondrial RNA; 2) nuclear receptor and coactivator/corepressor interactions, and; 3) the signal sequence in mitochondrial intermembrane space. These probes are generally applicable to fundamental biological studies as well as for assaying and screening possible pharmaceutical or toxic chemicals that facilitate or inhibit cellular signaling pathways.


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